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首页> 外文期刊>Electroanalysis >Employment of electrochemical techniques for metallothionein determination in tumor cell lines and patients with a tumor disease
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Employment of electrochemical techniques for metallothionein determination in tumor cell lines and patients with a tumor disease

机译：使用电化学技术测定肿瘤细胞系和肿瘤患者中的金属硫蛋白

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In the present paper we employed adsorptive transfer stripping technique coupled with chronopotentiometric stripping analysis for determination of metallothionein (MT) in tumor cell lines and differential pulse voltammetry Brdicka reaction for determination of MT in blood serum of patients with head and neck cancer or retinoblastoma, and of rats treated with cisplatin with respect to discuss the role of MT in formation of resistance on treatment with heavy metal based cytostatics. The cisplatin or carboplatin sensitive and resistant neuroblastoma cell lines were derived from the maternal cell line isolated from the bone metastasis of patients with neuroblastoma. Based on the results obtained it can be concluded that level of MT increases with higher dose of platinum based cytostatics at cells. Further we focused on determination of MT in blood serum of rats treated with cisplatin (two doses 1.05 mg and/or 2.1 mg of cisplatin per kg). The highest level of MT at rats treated with 1.05 mg cisplatin was determined after four hours as 4.9 mu mol/L. In the case of the second experimental group the maximum was reached even after two hours of the treatment as 4.8 mu mol/L. In addition we were interested in the effect of cisplatin or carboplatin treatment of patients with a tumor disease. At patients with tumor in head and neck area treated with cisplatin we observed that the level of MT was going higher due to administration of the drug. This phenomenon was observed at all patients. However at patients with retinoblastoma treated with carboplatin we observed various phenomena including decreasing, increasing or no changes in MT level. Progression of MT levels was therefore individual and probably depended on tumor resistance to carboplatin.
机译：在本文中，我们采用吸附转移溶出技术结合计时电位溶出分析法测定肿瘤细胞系中的金属硫蛋白（MT），差示脉冲伏安法Brdicka反应测定头颈部癌或视网膜母细胞瘤患者血清中的MT，以及顺铂治疗的大鼠，探讨MT在重金属基细胞抑制剂治疗中形成耐药性的作用。顺铂或卡铂敏感和耐药的神经母细胞瘤细胞系衍生自母体细胞系，该母体细胞系分离自神经母细胞瘤患者的骨转移。根据获得的结果，可以得出结论，MT的水平随着细胞中铂基细胞抑制剂的剂量增加而增加。此外，我们专注于测定用顺铂治疗的大鼠血清中的MT（两种剂量每公斤1.05 mg和/或2.1 mg顺铂）。四小时后确定用1.05 mg顺铂处理的大鼠的MT最高水平为4.9μmol / L。在第二实验组的情况下，即使在处理两个小时后也达到了4.8μmol/ L的最大值。此外，我们对顺铂或卡铂治疗肿瘤患者的疗效感兴趣。在用顺铂治疗的头颈部肿瘤患者中，我们观察到由于服用该药物，MT水平正在升高。在所有患者中均观察到这种现象。但是，在接受卡铂治疗的成视网膜细胞瘤患者中，我们观察到各种现象，包括MT水平降低，升高或没有变化。因此，MT水平的进展是个体的，可能取决于肿瘤对卡铂的耐药性。

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