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Amperometric enzyme-based biosensor for direct detection of formaldehyde in the gas phase: Dependence on electrolyte composition

机译：用于直接检测气相甲醛的基于安培酶的生物传感器：取决于电解质成分

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摘要

An enzymatic sensor detecting the analyte formaldehyde directly from the gas phase is under investigation. In contrast to existing systems, it enables the quantification of the analyte without prior sampling or accumulation and thus can be used as an online system to monitor the formaldehyde concentration in ambient air. The amperometric sensor depends on the enzymatic conversion of the analyte using formaldehyde dehydrogenase from P putida [EC. 1.2.1.46] as the recognition element. It shows a linear response curve up to 15 ppm, with a detection limit of 0.03 pm (S/N= 3). In order to optimize the sensor performance the electrolyte composition within the sensor was varied with respect to pH value, buffer concentration and the addition of Ca2+ and Mg2+ ions. To elucidate the influence of the mediator and the enzyme on the sensor performance the stability and activity of the electrochemical mediator and the enzyme alone was examined separately in these different electrolytes.
机译：正在研究一种直接从气相中检测被分析物甲醛的酶传感器。与现有系统相比，它无需进行事先采样或积累即可定量分析物，因此可以用作在线系统来监测环境空气中的甲醛浓度。安培传感器取决于使用恶臭假单胞菌的甲醛脱氢酶[EC。 1.2.1.46]作为识别元素。它显示了高达15 ppm的线性响应曲线，检出限为0.03 pm（S / N = 3）。为了优化传感器性能，传感器内的电解质成分会根据pH值，缓冲液浓度以及Ca2 +和Mg2 +离子的添加​​而变化。为了阐明介体和酶对传感器性能的影响，在这些不同的电解质中分别检查了电化学介体和酶的稳定性和活性。

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