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首页> 外文期刊>Endothelium: Journal of endothelial cell research >Vascular endothelial growth factor in porcine-derived extracellular matrix.
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Vascular endothelial growth factor in porcine-derived extracellular matrix.

机译：猪源性细胞外基质中的血管内皮生长因子。

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摘要

An extracellular matrix (ECM) derived from the submucosa of the porcine small intestine (SIS) has been shown to induce angiogenesis and host tissue remodeling when used as a xenogeneic bioscaffold in animal models of wound repair. In the present study, we compared the in vitro effects of SIS ECM extracts to several purified angiogenic growth factors on human dermal microvascular endothelial cell (HMEC) growth patterns. The SIS ECM was shown to induce tube formation from HMEC in a three-dimensional fibrin-based angiogenesis assay in a manner similar to that caused by the addition of vascular endothelial growth factor (VEGF). This tube formation was blocked in the presence of anti-VEGF neutralizing antibody. Western blots and ELISA procedures showed that the SIS ECM contains as much as 0.77 ng VEGF/g SIS. The closely related endothelial cell mitogen, platelet-derived growth factor (PDGF), was not detectable in the SIS extracts. We conclude that VEGF is present in the SIS extracellular matrix. The role of VEGF in SIS-induced wound repair remains unknown, but its presence in the ECM makes it a possible contributor to the angiogenic effect of SIS when this ECM is used as a tissue repair scaffold in animal models of wound repair.
机译：当在伤口修复的动物模型中用作异种生物支架时，已证明衍生自猪小肠（SIS）粘膜下层的细胞外基质（ECM）诱导血管生成和宿主组织重塑。在本研究中，我们比较了SIS ECM提取物与几种纯化的血管生成生长因子对人皮肤微血管内皮细胞（HMEC）生长模式的体外作用。在基于纤维蛋白的三维血管生成测定中，SIS ECM被证明可诱导HMEC形成管，其方式类似于添加血管内皮生长因子（VEGF）所引起的方式。在抗VEGF中和抗体的存在下，该管的形成被阻断。 Western印迹和ELISA程序显示SIS ECM含有多达0.77 ng VEGF / g SIS。在SIS提取物中未检测到密切相关的内皮细胞有丝分裂原，即血小板衍生的生长因子（PDGF）。我们得出结论，在SIS细胞外基质中存在VEGF。 VEGF在SIS诱导的伤口修复中的作用仍然未知，但是当该ECM在伤口修复的动物模型中用作组织修复支架时，其在ECM中的存在使其可能成为SIS的血管生成作用的原因。

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