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首页> 外文期刊>Endothelium: Journal of endothelial cell research >Influence of several methodological procedures utilized to obtain in vitro vascular preparations on endothelial activity.
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Influence of several methodological procedures utilized to obtain in vitro vascular preparations on endothelial activity.

机译：用于获得体外血管制剂的几种方法学程序对内皮活性的影响。

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摘要

Several maneuvers usually employed to set up isolated vascular preparations could effect the endothelium-dependent relaxation (EDR). The effects of five such maneuvers were studied in rings of rat aorta: 1) Type of anesthesia, 2) Cold storage of the vessels, 3) Length of the stabilization period, 4) Repeated contractions during stabilization, and 5) Performance of washouts during stabilization. Repeated contractions with norepinephrine (NE) 0.1 microM after stabilization altered neither the contraction nor the EDR induced by acetylcholine (Ach) 1 microM. Pentobarbital anesthesia and cold storage of the preparations for 24 h significantly decreased the EDR without effecting the contractile response of the rings. The absence of washouts during stabilization increased the contractions to either NE 0.1 microM or KCl 80 mM by nearly 50%. This increase was prevented by endothelial disruption or, in the presence of intact endothelium, by repeated washouts or by incubation with Bosentan 22 microM. It is concluded that 1) Anesthesia of the animals and cold storage of the preparations can alter the EDR even in the absence of contractile changes in the smooth muscle, and 2) Accumulation of endothelin during the incubation period, even if not producing changes in the resting tension, can substantially alter the subsequent response to vasoactive interventions.
机译：通常用于建立孤立的血管制剂的几种操作可能会影响内皮依赖性舒张（EDR）。在大鼠主动脉环中研究了五种这样的操作的效果：1）麻醉的类型，2）血管的冷藏，3）稳定期的长度，4）稳定过程中反复收缩，以及5）冲洗过程中的冲洗性能稳定。稳定后，用去甲肾上腺素（NE）0.1 microM反复收缩不会改变收缩或乙酰胆碱（Ach）1 microM引起的EDR。戊巴比妥麻醉和制剂冷藏24小时可显着降低EDR，而不会影响环的收缩反应。稳定过程中不存在冲刷作用，使收缩至NE 0.1 microM或KCl 80 mM增加了近50％。通过内皮破坏或在存在完整内皮的情况下通过反复冲洗或与Bosentan 22 microM孵育可防止这种增加。结论是：1）动物的麻醉和制品的冷藏可以改变EDR，即使在平滑肌中没有收缩变化的情况下，也可以2）在温育期内皮素的积累，即使在体内不会产生变化。静息紧张状态可以大大改变对血管活性干预措施的后续反应。

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