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首页> 外文期刊>Endothelium: Journal of endothelial cell research >Unique sensitivities to cytokine regulated expression of adhesion molecules in human heart-derived endothelial cells.
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Unique sensitivities to cytokine regulated expression of adhesion molecules in human heart-derived endothelial cells.

机译：对人心脏来源的内皮细胞中细胞因子调节粘附分子表达的独特敏感性。

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The expression of adhesion molecules by endothelial cells is crucial in many inflammatory processes and plays an active role in the development of reperfusion injury, acute and chronic rejection. The expression of adhesion molecules in different parts of the coronary tree to cytokine stimulation is not known. We describe here a detailed study of the effects of the inflammatory cytokines TNFalpha and IL-1beta on the expression of adhesion molecules vascular cell adhesion molecule-1 (VCAM-1), E-selectin and intracellular cell adhesion molecule-1 (ICAM-1) on human aortic root (HAEC), coronary artery (HCAEC) and heart microvascular (HHMEC)) endothelial cells in culture, using flow cytometry. We found constitutive levels of both VCAM-1 and E-Selectin on HCAEC and HHMEC (approximately 20%) which were significantly higher compared to HAEC (approximately 3%). There was an extreme sensitivity of HCAEC and HHMEC to 0.002 ng/ml TNFalpha: (VCAM-1 approximately 40%, E-Selectin approximately 25%) respectively, compared to HAEC (VCAM-1 approximately 5%, E-selectin approximately 5%). IL-1beta showed a similar pattern of expression at low doses (5 U/ml), but was less potent. We also observed prolonged expression of these adhesion molecules, especially on the HHMEC (>48 hours) compared to HAEC. There was also increased binding of peripheral blood mononuclear cells (PBMC) to both non-stimulated and TNFalpha stimulated HCAEC and HHMEC compared to HAEC. This data suggest that endothelial cells in different regions of the coronary tree express different patterns of basal and cytokine-stimulated adhesion molecule expression.
机译：内皮细胞粘附分子的表达在许多炎症过程中至关重要，并在再灌注损伤，急性和慢性排斥反应的发生中起积极作用。粘附分子在冠状树不同部位对细胞因子刺激的表达尚不清楚。我们在此描述炎性细胞因子TNFalpha和IL-1beta对粘附分子血管细胞粘附分子1（VCAM-1），E-选择素和细胞内细胞粘附分子1（ICAM-1）表达的影响的详细研究使用流式细胞仪在培养的人主动脉根（HAEC），冠状动脉（HCAEC）和心脏微血管（HHMEC））内皮细胞上）我们发现HCAEC和HHMEC上的VCAM-1和E-选择素的组成水平（约20％）明显高于HAEC（约3％）。与HAEC（VCAM-1约5％，E-选择素约5％）相比，HCAEC和HHMEC对0.002 ng / ml TNFalpha的极度敏感性：（VCAM-1约40％，E-选择素约25％） ）。 IL-1beta在低剂量（5 U / ml）下表现出相似的表达模式，但效力较低。我们还观察到与HAEC相比，这些黏附分子的表达延长，尤其是在HHMEC上（> 48小时）。与HAEC相比，外周血单核细胞（PBMC）与非刺激和TNFalpha刺激的HCAEC和HHMEC的结合也增加。该数据表明，冠状动脉树的不同区域中的内皮细胞表达基础和细胞因子刺激的粘附分子表达的不同模式。

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