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首页> 外文期刊>Endothelium: Journal of endothelial cell research >Down-regulation of endothelial ephrinB2 expression by laminar shear stress.
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Down-regulation of endothelial ephrinB2 expression by laminar shear stress.

机译：层状切应力下调内皮ephrinB2的表达。

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摘要

The EphB receptors and their ephrinB ligands are involved in vascular assembly and differentiation. In this study, the authors analyzed the regulation of ephrinB2 and EphB4 in response to laminar shear stress in human endothelial cells. In order to simulate different flow conditions in vitro, human endothelial cells were exposed to laminar shear stress (1 to 50 dyn/cm2 for up to 24 h) in a cone-and-plate viscometer. EphrinB2 mRNA expression is down-regulated by arterial, but not by venous, laminar shear stress in a dose-dependent manner in primary cultures of human umbilical vein endothelial cells (HUVECs) (maximum at 30 dyn/cm2, 24 h: 46% +/- 4%of internal control without shear stress, n = 16, p < .05). The down-regulation of ephrinB2 by arterial shear stress is blocked by the protein kinase C inhibitor RO-31-8220. A similar shear stress-dependent down-regulation of ephrin-B2 can be found in human coronary artery endothelial cells (HCAECs). Chronic application of laminar shear stress does not affect EphB4 expression in venous and arterial endothelial cells. The down-regulation of ephrinB2 in response to laminar shear stress may contribute to the differentiation of endothelial cells into a nonactivated phenotype.
机译：EphB受体及其ephrinB配体参与血管组装和分化。在这项研究中，作者分析了响应人内皮细胞层流切应力的ephrinB2和EphB4的调节。为了模拟体外不同的流动条件，在锥板粘度计中将人内皮细胞暴露于层流剪切应力（1至50 dyn / cm2，长达24小时）。在人脐静脉内皮细胞（HUVEC）的原代培养中，EphrinB2 mRNA表达受动脉但不受到静脉，层流切应力的剂量依赖性下调（最大30 dyn / cm2，24 h：46％+ /-内部控制的4％，没有剪切应力，n = 16，p <.05）。蛋白激酶C抑制剂RO-31-8220阻止了动脉剪切应力对ephrinB2的下调。在人冠状动脉内皮细胞（HCAEC）中可以发现类似的依赖于切应力的ephrin-B2下调。长期施加层流切应力不会影响EphB4在静脉和动脉内皮细胞中的表达。响应层流切应力的ephrinB2下调可能有助于内皮细胞分化为非活化表型。

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