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首页> 外文期刊>Electromagnetic biology and medicine >Effects of 50 Hz electromagnetic fields on the histology, apoptosis, and expression of c-Fos and β-Catenin on the livers of preincubated white leghorn chicken embryos
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Effects of 50 Hz electromagnetic fields on the histology, apoptosis, and expression of c-Fos and β-Catenin on the livers of preincubated white leghorn chicken embryos

机译：50 Hz电磁场对预培养的白色来亨鸡雏鸡肝脏组织学，细胞凋亡以及c-Fos和β-Catenin表达的影响

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摘要

Reports have demonstrated occurrences of abnormalities in the early stages of chicken embryonic development due to the exposure to electromagnetic fields (EMFs). This article was designed to investigate the effects of sinusoidal EMF on the histopathology, apoptosis, and expressions of c-Fos and β-Catenin genes of the livers of preincubated White Leghorn chicken embryos, based on our published experiments. 300 healthy, fresh fertilized eggs were divided into control (n = 70), sham (n = 70), and four experimental (1 -4,days 13, 14, 5, and 19, n = 40) groups. Experimental eggs were exposed to the most effective intensity in a coil with 7.32 mT density, and sham groups were also located in the same coil with no exposure, both for 24 h before incubation. Control, sham, and experimental groups were then incubated in an incubator (37°C, humidity 60%) for 13,14,15, and 19 days. Livers of 13-15 and 19 day-old chicken embryos were removed by C-section and fixed in formalin (10%), stained with Hematoxylin-Eosin and TUNEL for histopathological and apoptosis studies. Others were used for investigating c-Fos and 3-Catenin expressions, using RT-PCR. Results showed extensive hemorrhages all over the chicken embryos' bodies and livers, more lymphoid tissues, disturbed parenchymal tissues, sinusoid denaturation, vesiculizad cytoplasm, an increase in the number of apoptotic cells, and a decrease on the levels of expressions of c-Fos and β-Catenin genes in experimental groups of 1-4, comparing control and sham groups.
机译：有报告表明，由于暴露于电磁场（EMF），导致鸡胚胎发育早期出现异常。本文旨在根据我们已发表的实验研究正弦电动势对预培养的白色来格霍恩鸡胚胎肝脏组织病理学，细胞凋亡以及c-Fos和β-Catenin基因表达的影响。将300个健康的新鲜受精卵分为对照组（n = 70），假（n = 70）和四个实验组（1-4天，第13、14、5和19天，n = 40）。将实验鸡蛋在密度为7.32 mT的线圈中暴露于最有效强度，并且在孵化之前，将假手术组也置于相同线圈中且没有暴露，时间均为24 h。然后将对照组，假手术组和实验组在培养箱（37°C，湿度60％）中培养13、14、15和19天。通过剖腹术取出13-15和19天大的鸡胚胎的肝脏，固定在福尔马林（10％）中，用苏木精-伊红和TUNEL染色以进行组织病理学和细胞凋亡研究。其他人则使用RT-PCR研究c-Fos和3-Catenin的表达。结果显示，整个鸡胚的身体和肝脏都大量出血，淋巴组织增多，实质组织受损，窦样变性，囊泡性细胞质，凋亡细胞数量增加，c-Fos和c-Fos表达水平降低。 1-4个实验组中的β-连环蛋白基因，比较对照组和假组。

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