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首页> 外文期刊>BioFactors >Preparation of morusin from Ramulus mori and its effects on mice with transplanted H-22 hepatocarcinoma
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Preparation of morusin from Ramulus mori and its effects on mice with transplanted H-22 hepatocarcinoma

机译：家蚕毛柔毛素的制备及其对H-22肝癌移植小鼠的影响

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In this study, we used branches Ramulus mori from cultivated mulberry Husang-32 (Morus multicaulis Perry) as the experimental material and anhydrous ethanol as the extraction solution to obtain the crude extract from the branch bark. The ethanolic extract was successively purified through a macroporous resin, Sephadex LH-20, and semipreparative reversed-phase high-performance liquid chromatography (RP-HPLC). The high-purity monomer was identified as morusin by HPLC with diode array detection (HPLC-DAD) and its UV spectrum. The contents of morusin exhibited almost no difference between the root and branch bark in Husang-32, and morusin was not detected in the leaves. Morusin is able to inhibit the tumor growth of transplanted H-22 hepatocarcinoma in mice and has no side effects. The fluorescence quantitative real-time PCR (qRT-PCR) results indicate that morusin has a marked inhibitory effect on liver cancer cells through a mechanism that may be related increases in the expression of p53, Survivin, CyclinB1, and Caspase-3 and a decrease in NF- B gene expression. The influence of this compound is more apparent in the Caspase-3 and the NF- B genes. (c) 2014 BioFactors, 40(6):636-645, 2014
机译：在这项研究中，我们使用栽培桑树Husang-32（桑属多汁桑树）的枝条毛ulu作为实验材料，无水乙醇作为提取液，从枝条皮中提取粗提物。通过大孔树脂Sephadex LH-20和半制备反相高效液相色谱（RP-HPLC）依次纯化乙醇提取物。通过具有二极管阵列检测（HPLC-DAD）的HPLC和其UV光谱，高纯度单体被鉴定为morusin。在Husang-32中，morusin的含量在根和分支皮之间几乎没有差异，并且在叶子中未检测到morusin。桑树素能够抑制小鼠移植的H-22肝癌的肿瘤生长，并且没有副作用。实时荧光定量PCR（qRT-PCR）结果表明，莫鲁辛通过与p53，Survivin，CyclinB1和Caspase-3表达增加相关的机制对肝癌细胞具有明显的抑制作用。在NF-B基因表达中。该化合物的影响在Caspase-3和NF-B基因中更为明显。 （c）2014生物因子，40（6）：636-645，2014

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