• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Equine Veterinary Journal >Seminal parameters and field fertility of cryopreserved donkey jack semen after insemination of horse mares
【24h】

Seminal parameters and field fertility of cryopreserved donkey jack semen after insemination of horse mares

机译：母马授精后低温保存的驴杰克精液的精液参数和田间肥力

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

P>Reasons for performing study:As mule production is often concentrated in remote areas of the world, a simplified semen cryopreservation protocol is required.Aim:To compare the seminal parameters of cryopreserved donkey semen in lactose-EDTA and lactose-yolk extenders and the fertility rates on horse mares.Methods:Trial 1: Sperm total and progressive motility, vigour (scale 0-5), morphology (major and minor defects) and plasma membrane integrity (HOST) were evaluated in 25 ejaculates from 5 donkey jacks immediately after collection (raw), after chilling to 5 degrees C (chilled) and after freezing/thawing. The semen was mixed with skimmed-milk extender, centrifuged, and then re-suspended in lactose-EDTA or lactose-yolk extender. Semen was loaded into 0.5 ml straws and chilled to 5 degrees C for 1 h, after which samples were either evaluated (chilled semen) or placed above liquid nitrogen for 20 min prior to immersion. Seminal parameters were evaluated by ANOVA and Tukey's test. Trial 2: Cryopreserved semen from 3 males was used to inseminate 53 mares at 60 oestrous cycles randomly assigned to lactose-yolk (n = 30 cycles) or lactose-EDTA (n = 30 cycles) extenders. Pregnancy diagnosis was performed 15 and 25 days post ovulation. The pregnancy rates were compared using Chi-squared tests.Results:Trial 1: No significant differences were evident in any seminal parameters between extenders after either chilling or cryopreservation. Total and progressive motility were significantly (P < 0.05) lower in cryopreserved semen than raw and chilled semen for both extenders. Trial 2: Pregnancy rates did not significantly differ between extenders (lactose-EDTA extender 53.33 and 43.33%; lactose-yolk 50.0 and 46.66% for Days 15 and 25 post ovulation, respectively).Conclusions:Cryopreservation of donkey semen using the simplified lactose-yolk extender resulted in similar seminal parameters and fertility rates when compared to lactose-EDTA extender.Potential relevance:Lactose-yolk extender may be advocated as a simple, easy to prepare extender, for use in geographically isolated enterprises producing mules throughout the world.
机译：P>进行研究的原因：由于m子的生产通常集中在世界偏远地区，因此需要简化的精液冷冻保存方案。目的：比较乳糖-EDTA和乳糖-蛋黄补充剂中冷冻保存的驴精液的主要参数。方法：试验1：在之后立即对5只驴子的25只精液中的精子进行总活力和进行性运动，活力（0-5级），形态（主要和次要缺陷）和质膜完整性（HOST）评估。冷却至5摄氏度（冷却）并冷冻/解冻后收集（原始）。将精液与脱脂乳补充剂混合，离心，然后重悬于乳糖-EDTA或乳糖-蛋黄补充剂中。将精液装入0.5 ml的吸管中，冷却至5摄氏度，保持1 h，然后评估样品（冷却的精液）或在浸入之前将其置于液氮上方20分钟。通过ANOVA和Tukey检验评估精液参数。试验2：使用来自3个雄性的冷冻保存的精液在随机分配给乳糖-蛋黄（n = 30个周期）或乳糖-EDTA（n = 30个周期）补充剂的60个发情周期内授精53匹母马。排卵后15和25天进行妊娠诊断。结果：试验1：冷藏或冷冻保存后，补充剂之间的任何精液参数均无明显差异。对于两种补充剂，冷冻精液中的总活力和进行性活力显着低于生精和冷藏精液。试验2：补充剂之间的怀孕率没有显着差异（排卵后第15天和第25天，乳糖-EDTA补充剂分别为53.33％和43.33％;乳糖-蛋黄分别为50.0和46.66％）。结论：使用简化的乳糖-冷冻保存驴精液与乳糖-EDTA补充剂相比，蛋黄补充剂产生的精液参数和受精率相似。潜在意义：乳糖-蛋黄补充剂可能被认为是一种简单易制备的补充剂，适用于在世界各地生产mu子的地域偏远的企业。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号