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首页> 外文期刊>Epigenetics: official journal of the DNA Methylation Society >Correlation of DNA methylation levels in blood and saliva DNA in young girls of the LEGACY Girls study
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Correlation of DNA methylation levels in blood and saliva DNA in young girls of the LEGACY Girls study

机译：LEGACY Girls研究的年轻女孩血液和唾液中DNA甲基化水平的相关性

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Many epidemiologic studies of environmental exposures and disease susceptibility measure DNA methylation in white blood cells (WBC). Some studies are also starting to use saliva DNA as it is usually more readily available in large epidemiologic studies. However, little is known about the correlation of methylation between WBC and saliva DNA. We examined DNA methylation in three repetitive elements, Sat2, Alu, and LINE-1, and in four CpG sites, including AHRR (cg23576855, cg05575921), cg05951221 at 2q37.1, and cg11924019 at CYP1A1, in 57 girls aged 6-15 years with blood and saliva collected on the same day. We measured all DNA methylation markers by bisulfite-pyrosequencing, except for Sat2 and Alu, which were measured by the MethyLight assay. Methylation levels measured in saliva DNA were lower than those in WBC DNA, with differences ranging from 2.8% for Alu to 14.1% for cg05575921. Methylation levels for the three repetitive elements measured in saliva DNA were all positively correlated with those in WBC DNA. However, there was a wide range in the Spearman correlations, with the smallest correlation found for Alu (0.24) and the strongest correlation found for LINE-1 (0.73). Spearman correlations for cg05575921, cg05951221, and cg11924019 were 0.33, 0.42, and 0.79, respectively. If these findings are replicated in larger studies, they suggest that, for selected methylation markers (e.g., LINE-1), methylation levels may be highly correlated between blood and saliva, while for others methylation markers, the levels may be more tissue specific. Thus, in studies that differ by DNA source, each interrogated site should be separately examined in order to evaluate the correlation in DNA methylation levels across DNA sources.
机译：环境暴露和疾病易感性的许多流行病学研究都测量了白细胞（WBC）中的DNA甲基化。一些研究也开始使用唾液DNA，因为在大型流行病学研究中通常更容易获得唾液DNA。但是，关于WBC和唾液DNA之间甲基化的相关性知之甚少。我们在57位6至15岁的女孩中检查了三个重复元件Sat2，Alu和LINE-1以及四个CpG位点的DNA甲基化，包括AHRR（cg23576855，cg05575921），在2q37.1处的cg05951221和在CYP1A1处的cg11924019在四个CpG位点。年收集同一天的血液和唾液。我们通过亚硫酸氢盐-焦磷酸测序法测量了所有DNA甲基化标记物，但Sat2和Alu除外，后者通过MethyLight测定法进行了测量。唾液DNA中测得的甲基化水平低于WBC DNA中的甲基化水平，差异范围从Alu的2.8％到cg05575921的14.1％。唾液DNA中三个重复元素的甲基化水平与WBC DNA中的甲基化水平均呈正相关。但是，Spearman相关性的范围很广，其中Alu的相关性最小（0.24），LINE-1的相关性最大（0.73）。 cg05575921，cg05951221和cg11924019的Spearman相关性分别为0.33、0.42和0.79。如果在较大的研究中重复这些发现，则表明对于选定的甲基化标记物（例如LINE-1），血液和唾液之间的甲基化水平可能高度相关，而对于其他甲基化标记物，该水平可能更具组织特异性。因此，在不同DNA来源的研究中，应分别检查每个被询问的位点，以评估跨DNA来源的DNA甲基化水平的相关性。

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