...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Epigenetics: official journal of the DNA Methylation Society >Individual variation and longitudinal pattern of genome-wide DNA methylation from birth to the first two years of life
【24h】

Individual variation and longitudinal pattern of genome-wide DNA methylation from birth to the first two years of life

机译：从出生到生命的前两年，全基因组DNA甲基化的个体差异和纵向模式

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Prenatal development and early childhood are critical periods for establishing the tissue-specific epigenome, and may have a profound impact on health and disease in later life. However, epigenomic profiles at birth and in early childhood remain largely unexplored. The focus of this report is to examine the individual variation and longitudinal pattern of genome-wide DNA methylation levels from birth through the first two years of life in 105 Black children (59 males and 46 females) enrolled at the Boston Medical Center. We performed epigenomic mapping of cord blood at birth and venous blood samples from the same set of children within the first two years of life using Illumina Infinium Humanmethylation27 BeadChip. We observed a wide range of inter-individual variations in genome-wide methylation at each time point including lower levels at CpG islands, TSS 200, 5' UTR and 1st Exon locations, but significantly higher levels in CpG shores, shelves, TSS 1500, gene body and 3' UTR. We identified CpG sites with significant intra-individual longitudinal changes in the first two years of life throughout the genome. Specifically, we identified 159 CpG sites in males and 149 CpG sites in females with significant longitudinal changes defined by both statistical significance and magnitude of changes. These significant CpG sites appeared to be located within genes with important biological functions including immunity and inflammation. Further studies are needed to replicate our findings, including analysis by specific cell types, and link those individual variations and longitudinal changes with specific health outcomes in early childhood and later life.
机译：产前发育和儿童早期是建立组织特异性表观基因组的关键时期，可能对以后的健康和疾病产生深远影响。然而，在出生时和儿童早期的表观基因组概况仍未得到充分探索。本报告的重点是研究在波士顿医学中心招募的105名黑人儿童（男59例，女46例）从出生到出生的前两年的全基因组DNA甲基化水平的个体差异和纵向模式。我们使用Illumina Infinium Humanmethylation27 BeadChip在出生后的头两年内对同一组儿童的出生时的脐血和静脉血样本进行了表观基因组学定位。我们观察到每个时间点的全基因组甲基化范围各不相同，包括CpG岛，TSS 200、5'UTR和1st Exon位置的水平较低，而CpG岸，架子，TSS 1500，基因体和3'UTR。我们确定了在整个基因组生命的头两年中，个体内部纵向发生重大变化的CpG位点。具体而言，我们确定了男性中的159个CpG位点和女性中的149个CpG位点，其纵向显着变化由统计学意义和变化幅度定义。这些重要的CpG位点似乎位于具有重要生物学功能（包括免疫力和炎症）的基因内。需要进一步的研究来复制我们的发现，包括按特定细胞类型进行分析，并将这些个体差异和纵向变化与儿童早期和晚年的特定健康结果联系起来。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号