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首页> 外文期刊>Brazilian Archives of Biology and Technology >Quantitative Differential Expression of Alpha and Beta Ryanodine Receptor Genes in PSE (Pale, Soft, Exudative) Meat from Two Chicken Lines: Broiler and Layer
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Quantitative Differential Expression of Alpha and Beta Ryanodine Receptor Genes in PSE (Pale, Soft, Exudative) Meat from Two Chicken Lines: Broiler and Layer

机译：两种鸡品系（肉鸡和蛋鸡）的PSE（淡，软，渗出）肉中α和βRyanodine受体基因的定量差异表达

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摘要

Total RNA isolated from Pectoralis major muscle from PSE (L-24h*>53.0. pH<5.8) and non-PSE (44 <= L-24h*>= 53) meats of two phenotypically distinct chicken lines, broiler and layer, was used to investigate the alpha-ryr and beta-ryr gene expression by real-time RT-PCR approach. Mean relative quantification (RQ) values were lower (p<0.05) for beta-ryr in PSE chickens from both lines when compared to non-PSE chickens, while there was no difference (p>0.05) in alpha-tyr gene expression regardless of line studied. The beta-ryr RQ results suggested that in PSE samples an alteration might occur in the regular ratio (1:1) of alpha-RyR/beta-RyR normally found in avian muscles. These results provided the first evidence of PSE meat occurrence as a result of the differential expression of ryanodine receptor genes which might lead to an increased in Ca2+ availability at the cell milieu.
机译：从两个表型不同的鸡种，肉鸡和蛋鸡的PSE（L-24h *> 53.0。pH <5.8）和非PSE（44 <= L-24h *> = 53）肉的胸大肌分离的总RNA为用于通过实时RT-PCR方法研究alpha-ryr和beta-ryr基因表达。与非PSE鸡相比，两种品系的PSE鸡中β-ryr的平均相对定量（RQ）值均较低（p <0.05），而无论如何，α-tyr基因表达均无差异（p> 0.05）行研究。 β-ryrRQ结果表明，在PSE样品中，通常在禽类肌肉中发现的alpha-RyR / beta-RyR的规则比例（1：1）可能发生变化。这些结果提供了PSE肉出现的第一个证据，这是由于ryanodine受体基因的差异表达可能会导致细胞环境中Ca2 +的利用率增加。

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