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首页> 外文期刊>Biochimica et biophysica acta. Gene structure and expression >Regulated tRNA import in Leishmania mitochondria
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Regulated tRNA import in Leishmania mitochondria

机译：利什曼原虫线粒体中受调控的tRNA导入

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摘要

The genes for three new tRNA and a 5S RNA were identified from a genomic DNA clone of 917 nucleotide pairs from the protozoon Leishmania tarentolae. They were encoded in the following order. The transcriptional directions and anticodons are in parentheses: tRNA~(Val) (CAC →)-5SRNA (→)-tRNA~(His) (← GUG)-tRNA~(Phe) (GAA →). The tRNA~(His) and tRNA~(Phe) sequences have not been reported previously in trypanosomatid organisms. By northern analysis, tRNA~(Val) and tRNA~(Phe) were equally distributed between the cytosol and mitochondria, while tRNA~(His) was less abundant in mitochondria than in the cytosol. Accordingly, the latter tRNA is classified as Import restricted (Imp~r). As shown before, 5S RNA was not imported. Recently, Mahapatra and Adhya [S. Mahapatra, T. Ghosh, S. Adhya, Nucl. Acids Res. 22 (1994) 3381-3386; S. Mahapatra, S. Adhya, J. Biol. Chem. 271 (1996) 20432-20437] have developed an in vitro import system in Leishmania and suggested that the D-loop sequence could serve as the import determinant. We examined all available tRNA gene sequences in trypanosomatids but found no apparent consensus sequences within the D-loop that might account for tRNA-import regulation.
机译：从原生动物利什曼原虫塔伦托雷氏菌917个核苷酸对的基因组DNA克隆中鉴定出三个新的tRNA和5S RNA的基因。它们按以下顺序编码。转录方向和反密码子在括号中：tRNA〜（Val）（CAC→）-5SRNA（→）-tRNA〜（His）（←GUG）-tRNA〜（Phe）（GAA→）。 tRNA〜（His）和tRNA〜（Phe）序列以前在锥虫生物中尚未见报道。通过Northern分析，tRNA〜（Val）和tRNA〜（Phe）在胞质和线粒体之间均等分布，而tRNA〜（His）在线粒体中的丰富度小于在胞质中。因此，后一种tRNA被归类为进口受限（Imp_r）。如前所示，未导入5S RNA。最近，Mahapatra和Adhya [S. Mahapatra，T.Ghosh，S.Adhya，Nucl。酸Res。 22（1994）3381-3386; S. Mahapatra，S。Adhya，J。Biol。化学271（1996）20432-20437]在利什曼原虫中开发了一种体外导入系统，并建议D环序列可以作为导入决定因素。我们检查了锥虫中所有可用的tRNA基因序列，但未发现D环内有明显的共有序列可能解释tRNA导入调控。

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