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Effects of Organophosphorous Pesticides Used in China on Various Mammalian Cells

机译：中国使用的有机磷农药对哺乳动物细胞的影响

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Organophosphorous pesticides are currently widely used in China to help boost agricultural production.However,these pesticides pose various threats to organisms,including humans,and are thus a cause of concern.Five organophosphorous pesticides,monocrotophos,omethoate,parathion-methyl,phoxim and dichlorvos,were examined for their effects on mammalian cell lines to determine their potential impact on physiological functions in vivo.Results show an increased proliferation of MCF-7 cells treated with 0.2 muM monocrotophos or 0.4 muM omethoate,suggesting that these compounds can induce breast cancer cell proliferation at relatively low concentrations.Murine primary spleen cells markedly decreased in number starting at a pesticide concentration of 0.01 muM;no cytotoxicity was observed below 0.001 muM.BALB/c3T3 murine fibroblasts treated with 0.25 muM monocrotophos showed enhanced DNA synthesis,while those treated with the other pesticides showed results similar to that of the control.The different pesticides reduced the acetylcholinesterase(AChE)activity of the rat neuronal cell line PC 12 in a dose-dependent manner up to 100 muM.Parathion-methyl and phoxim showed acute toxicity at 0.01 muVl.Finally,phoxim and parathion-methyl significantly reduced the transepithelial electrical resistance(TEER)of human intestinal Caco-2 cells,indicating that these pesticides can disrupt the tight-junction permeability of cell monolayers.These in vitro assays,which are rapid,reproducible,simple and inexpensive,clearly show the effects of organophosphorous pesticides on mammalian cells and suggest the potential impact of these pesticides on organisms in vivo.
机译：目前，有机磷农药在中国已广泛用于促进农业生产。但是，这些农药对包括人类在内的生物体构成了各种威胁，因此引起了人们的关注。五种有机磷农药，久效磷，氧化乐果，对硫磷，甲基辛硫磷和敌敌畏研究了它们对哺乳动物细胞系的影响，以确定它们对体内生理功能的潜在影响。结果显示，用0.2μM久效磷或0.4μM氧化乐果处理的MCF-7细胞增殖增加，表明这些化合物可诱导乳腺癌细胞农药浓度为0.01μM时，鼠原代脾细胞数量明显减少;低于0.001μM时未观察到细胞毒性.0.25μM久效磷处理过的BALB / c3T3鼠成纤维细胞显示出增强的DNA合成，而其他农药的结果与对照相似。强大的杀虫剂以剂量依赖的方式降低了大鼠神经元细胞系PC 12的乙酰胆碱酯酶（AChE）活性。对硫磷和对硫磷在0.01μV时表现出急性毒性。最后，对硫磷和对硫磷显着降低了人肠道Caco-2细胞的跨上皮电阻（TEER），表明这些农药可破坏细胞单层的紧密连接通透性。这些体外测定快速，可重复，简单且廉价，清楚地显示了有机磷的作用杀虫剂对哺乳动物细胞的杀灭作用，并暗示了这些杀虫剂对体内生物的潜在影响。

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