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首页> 外文期刊>Enzyme and Microbial Technology >Interaction of small molecules with fungal laccase: A Surface Plasmon Resonance based study
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Interaction of small molecules with fungal laccase: A Surface Plasmon Resonance based study

机译：小分子与真菌漆酶的相互作用：基于表面等离子体共振的研究

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摘要

Laccases have a great potential for use in industrial and biotechnological applications. It has affinity towards phenolics and finds major applications in the field of bioremediation. Here, Surface Plasmon Resonance (SPR) as a biosensor with immobilized laccase on chip surface has been studied. Laccase was immobilized by thiol coupling method and compounds containing increasing number of hydroxyl groups were analyzed for their binding affinity at various concentrations in millimolar range. The small molecules like phloroglucinol (1.532 X 10(-8) M), crocin (3.204 x 10(-3) M), ascorbic acid (8.331 x 10(-8) M), kojic acid (6.411 x 10(-7) M) and saffron (3.466 x 10(-7) M) were studied and respective K-D values are obtained. The results were also confirmed by inhibition assay and IC50 values were calculated. All these molecules showed different affinity towards laccase in terms of K-D values. This method may be useful for preliminary screening and characterization of small molecules as laccase substrates, inhibitors or modulators of activity. This method will be useful for rapid screening of phenolics in waste water because of high sensitivity. (C) 2015 Elsevier Inc. All rights reserved.
机译：漆酶在工业和生物技术应用中具有巨大的潜力。它对酚醛树脂具有亲和力，并在生物修复领域找到了主要应用。在这里，已经研究了表面等离振子共振（SPR）作为在芯片表面固定有漆酶的生物传感器。通过硫醇偶联法固定漆酶，并分析了在毫摩尔范围内各种浓度下，羟基数目增加的化合物的结合亲和力。小分子如间苯三酚（1.532 X 10（-8）M），番红花（3.204 x 10（-3）M），抗坏血酸（8.331 x 10（-8）M），曲酸（6.411 x 10（-7） ）M）和藏红花（3.466 x 10（-7）M）进行了研究，并获得了各自的KD值。抑制试验也证实了结果，并计算了IC50值。就K-D值而言，所有这些分子对漆酶均显示出不同的亲和力。该方法可用于初步筛选和表征作为漆酶底物，活性抑制剂或调节剂的小分子。由于该方法灵敏度高，因此可用于快速筛选废水中的酚类。 （C）2015 Elsevier Inc.保留所有权利。

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