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首页> 外文期刊>Brazilian Archives of Biology and Technology >L-Arginine Metabolism in Mitochondria Isolated From the Liver of Antarctic Fish Notothenia rossii and Notothenia neglecta
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L-Arginine Metabolism in Mitochondria Isolated From the Liver of Antarctic Fish Notothenia rossii and Notothenia neglecta

机译：分离自南极鱼类罗氏线虫和疏忽线粒体肝脏的线粒体中的L-精氨酸代谢

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摘要

The arginase tissue distribution, the biochemical properties of the argininolytic system and the subcellular localization of the enzymes carbamoylphosphate svnthetase, ornithinecarbamoyl transierase, glutamine synthetase and arginase in Antarctic fish, N. neglecta and N. rossii were the main aims of the present work. The tissue with highest argininolytic activity was the kidney distal portion amounting as much as four times the specific activity of the hepatic tissue. Arginase and ornithine carbarnoyltransferase were found as mitochondrial enzymes, while glutamine synthetase and carbamoylphosphate synthetase were found as cytosolic enzymes. Argininolytic assays with isolated mitochondria gave values of K_(mapp) for the hydrolysis ofarginine 2 to 3.5times higher than the values found for the K,,, with mitochondrial extracts. The effect of Mn~(2+) on the argininolytic activity displayed by isolated mitochondria and mitochondrial extracts, in reaction conditions near the physiological ones showed that membranes were fundamentally involved in the control of L-arginine metabolism.
机译：南极鱼类，N。neglecta和N. rossii的精氨酸酶组织分布，精氨酸分解系统的生化特性以及氨基甲酰磷酸磷酸合酶，鸟氨酸氨基甲酰转氨酶，谷氨酰胺合成酶和精氨酸酶的亚细胞定位是本研究的主要目的。精氨酸分解活性最高的组织是肾脏远端部分，是肝组织比活性的四倍之多。发现精氨酸酶和鸟氨酸碳酰转移酶为线粒体酶，而谷氨酰胺合成酶和氨基甲酰磷酸合成酶为胞浆酶。分离线粒体的精氨酸分析法测定的精氨酸水解K_（mapp）值是线粒体提取物的K_（mapp）的K_（mapp）的值是K_（mapp）的两倍。 Mn〜（2+）对分离的线粒体和线粒体提取物所表现出的精氨酸分解活性的影响在生理条件附近的反应条件下表明，膜从根本上参与了L-精氨酸代谢的控制。

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