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首页> 外文期刊>European journal of nutrition >Influence of quercetin and rutin on growth and antioxidant defense system of a human hepatoma cell line (HepG2).
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Influence of quercetin and rutin on growth and antioxidant defense system of a human hepatoma cell line (HepG2).

机译：槲皮素和芦丁对人肝癌细胞系（HepG2）生长和抗氧化防御系统的影响。

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Background: Dietary polyphenols such as quercetin and rutin are considered beneficial because of their potential protective role in the pathogenesis of multiple diseases associated to oxidative stress, such as cancer, coronary heart disease and atherosclerosis. However, many of these effects may depend on the concentration of the polyphenol utilized since high doses of some phenolic compounds may be prooxidant and negatively affect cell growth and viability. Aim: To test the potential chemoprotective effects of quercetin and rutin, two flavonols with high antioxidant capacity, on the growth, viability, and response of the antioxidant defence system of a human hepatoma cell line (HepG2). Methods: Cell growth was measured by diaminobenzoic acid and bromodeoxyuridine assays, cell toxicity by lactate dehydrogenase leakage assay, reduced glutathione was quantified by a fluorimetric assay, cellular malondialdehyde was analysed by HPLC, reactive oxygen species were quantified by the dichlorofluorescein assay, antioxidant enzyme activities were determined by spectrophotometric analysis, and their gene expression by northern blot. Results: Short-term exposure (4 h) to these flavonols had no antiproliferative nor cytotoxic effect. High doses of quercetin (50-100 micro M) increased glutathione concentration and gene expression of Cu/Zn superoxide dismutase and catalase inhibiting the activity of the latter enzyme, whereas lower doses (0.1-1 micro M) decreased gene expression of Cu/Zn superoxide dismutase and increased that of glutathione peroxidase. All doses of quercetin and rutin diminished reactive oxygen species and high doses (10-100 micro M) decreased malondialdehyde concentration. Conclusion: The results indicate that both natural antioxidants induce favourable changes in the antioxidant defence system of cultured HepG2 that prevent or delay conditions that favour cellular oxidative stress..
机译：背景：槲皮素和芦丁等膳食多酚被认为是有益的，因为它们在与氧化应激相关的多种疾病（例如癌症，冠心病和动脉粥样硬化）的发病机理中具有潜在的保护作用。但是，这些作用中的许多作用可能取决于所用多酚的浓度，因为高剂量的某些酚类化合物可能是促氧化剂，会对细胞生长和生存能力产生负面影响。目的：为了测试槲皮素和芦丁（两种具有高抗氧化能力的黄酮醇）对人肝癌细胞系（HepG2）的生长，活力和抗氧化防御系统反应的潜在化学保护作用。方法：通过二氨基苯甲酸和溴脱氧尿苷测定法检测细胞生长，通过乳酸脱氢酶渗漏测定法测定细胞毒性，通过荧光测定法测定还原型谷胱甘肽，通过HPLC分析细胞丙二醛，通过二氯荧光素测定法测定活性氧，抗氧化酶活性通过分光光度分析确定它们的表达，并通过northern blot检测它们的基因表达。结果：短期接触这些黄酮醇（4小时）没有抗增殖或细胞毒性作用。高剂量槲皮素（50-100 micro M）会增加谷胱甘肽浓度和Cu / Zn超氧化物歧化酶和过氧化氢酶的基因表达，从而抑制后者的活性，而较低剂量（0.1-1 micro M）会降低Cu / Zn的基因表达。超氧化物歧化酶和增加谷胱甘肽过氧化物酶。槲皮素和芦丁的所有剂量均减少了活性氧的含量，高剂量（10-100 micro M）降低了丙二醛的浓度。结论：结果表明，两种天然抗氧化剂均能诱导培养的HepG2的抗氧化剂防御系统发生有利的变化，从而阻止或延迟有利于细胞氧化应激的条件。

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