Evaluation of the VITEK 2 AST-N111 card for detection of extended-spectrum beta-lactamases (ESBLs) in Escherichia coli, Klebsiella pneumoniae, and Klebsiella oxytoca compared to ESBL Etests and combination disk methods.

Valenza G; Muller S; Schmitt C; Turnwald D; Lam TT; Frosch M; Abele Horn M; Pfeifer Y

首页> 外文期刊>European journal of clinical microbiology and infectious diseases: Official publication of the European Society of Clinical Microbiology >Evaluation of the VITEK 2 AST-N111 card for detection of extended-spectrum beta-lactamases (ESBLs) in Escherichia coli, Klebsiella pneumoniae, and Klebsiella oxytoca compared to ESBL Etests and combination disk methods.

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Evaluation of the VITEK 2 AST-N111 card for detection of extended-spectrum beta-lactamases (ESBLs) in Escherichia coli, Klebsiella pneumoniae, and Klebsiella oxytoca compared to ESBL Etests and combination disk methods.

机译：评估VITEK 2 AST-N111卡与ESBL Etest和组合磁盘方法相比，可检测大肠埃希菌，肺炎克雷伯菌和产氧克雷伯菌中的广谱β-内酰胺酶（ESBL）。

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The VITEK 2 AST-N111 card was evaluated for detection of extended-spectrum beta-lactamases (ESBLs) by testing 51 ESBL positive and 50 ESBL negative isolates of E. coli, K. pneumoniae, and K. oxytoca. The occurrence of beta-lactamase genes was confirmed by PCR and sequencing. The advanced expert system (AES) of the VITEK 2 system achieved sensitivity and specificity values of 100% and 96.0%, respectively. The ESBL test of the VITEK 2 AST-N111 card showed a sensitivity of 92.1% and a specificity of 90.0%. Contradictory results obtained with the two VITEK 2 tools could be clarified by combination disk tests in nine of 11 isolates. The combined use of AES and ESBL tests of the AST-N111 card in association with combination disk tests in case of contradictory results seems to be a reliable method for ESBL detection.

机译：通过测试51株ESBL阳性，50株ESBL阴性的大肠杆菌，肺炎克雷伯菌和产氧克雷伯菌，对VITEK 2 AST-N111卡进行了广谱β-内酰胺酶（ESBLs）检测。通过PCR和测序证实了β-内酰胺酶基因的出现。 VITEK 2系统的高级专家系统（AES）的灵敏度和特异性值分别达到100％和96.0％。 VITEK 2 AST-N111卡的ESBL测试显示灵敏度为92.1％，特异性为90.0％。通过在11个分离物中的9个中进行组合磁盘测试，可以证明使用两个VITEK 2工具获得的矛盾结果。在结果矛盾的情况下，结合使用AST-N111卡的AES和ESBL测试以及磁盘组合测试似乎是一种可靠的ESBL检测方法。

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《European journal of clinical microbiology and infectious diseases: Official publication of the European Society of Clinical Microbiology》 |2011年第7期|共4页
作者
Valenza G; Muller S; Schmitt C; Turnwald D; Lam TT; Frosch M; Abele Horn M; Pfeifer Y;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Evaluation of the VITEK 2 AST-N111 card for detection of extended-spectrum beta-lactamases (ESBLs) in Escherichia coli, Klebsiella pneumoniae, and Klebsiella oxytoca compared to ESBL Etests and combination disk methods. [J] . Valenza G, Muller S, Schmitt C, European journal of clinical microbiology and infectious diseases: Official publication of the European Society of Clinical Microbiology . 2011,第7期

机译：评估VITEK 2 AST-N111卡与ESBL Etest和组合磁盘方法相比，可检测大肠埃希菌，肺炎克雷伯菌和产氧克雷伯菌中的广谱β-内酰胺酶（ESBL）。
2. Evaluation of the Etest ESBL and the BD Phoenix, VITEK 1, and VITEK 2 Automated Instruments for Detection of Extended-Spectrum Beta-Lactamases in Multiresistant Escherichia coli and Klebsiella spp. [J] . Maurine A. Leverstein-van Hall, Ad C. Fluit, Armand Paauw, Journal of Clinical Microbiology . 2002,第10期

机译：评估Etest ESBL和BD Phoenix，VITEK 1和VITEK 2自动化仪器，以检测多重耐药性大肠杆菌和克雷伯氏菌属中的广谱β-内酰胺酶。
3. Evaluation of detection of extended-spectrum beta-lactamases among Escherichia coli and Klebsiella spp. isolates by VITEK 2 AST-N029 compared to the agar dilution and disk diffusion methods. [J] . Nyberg SD, Meurman O, Jalava J, Scandinavian journal of infectious diseases. . 2008,第5期

机译：评价大肠埃希氏菌和克雷伯菌属中的广谱β-内酰胺酶。与琼脂稀释和圆盘扩散法相比，通过VITEK 2 AST-N029分离得到。
4. Characterization of putative extended-spectrum beta-lactamases (ESBL) producing Escherichia coli isolated from feedlot cattle in southern Alberta. [D] . Lussier, Pamela. 2010

机译：从艾伯塔省南部的肥育场牛分离出的推定广谱β-内酰胺酶（ESBL）大肠杆菌的表征。
5. Evaluation of the Etest ESBL and the BD Phoenix VITEK 1 and VITEK 2 Automated Instruments for Detection of Extended-Spectrum Beta-Lactamases in Multiresistant Escherichia coli and Klebsiella spp. [O] . Maurine A. Leverstein-van Hall, Ad C. Fluit, Armand Paauw, 2002

机译：评估Etest ESBL和BD PhoenixVITEK 1和VITEK 2自动化仪器以检测多重耐药性大肠杆菌和克雷伯氏菌属中的广谱β-内酰胺酶。
6. Evaluation of the VITEK 2 AST-N111 card for detection of extended-spectrum beta-lactamases (ESBLs) in Escherichia coli, Klebsiella pneumoniae, and Klebsiella oxytoca compared to ESBL Etests and combination disk methods [O] . G. Valenza, S. Müller, C. Schmitt, 2011

机译：与ESBL恒定和组合磁盘方法相比，对大肠杆菌，Klebsiella肺炎和Klebsiella oxytoca进行检测的Vitek 2 AST-N111卡的评估。

Evaluation of the VITEK 2 AST-N111 card for detection of extended-spectrum beta-lactamases (ESBLs) in Escherichia coli, Klebsiella pneumoniae, and Klebsiella oxytoca compared to ESBL Etests and combination disk methods.

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