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首页> 外文期刊>European journal of pain : >Automated measurement of spontaneous pain-associated limb movement and drug efficacy evaluation in a rat model of neuropathic pain.
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Automated measurement of spontaneous pain-associated limb movement and drug efficacy evaluation in a rat model of neuropathic pain.

机译：在神经性疼痛大鼠模型中自动测量与疼痛相关的自发性肢体运动并评估药效。

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The withdrawal response elicited by a nociceptive stimulus, i.e., evoked pain measure, is commonly used as an efficacy endpoint in neuropathic pain animal models. It, however, has several limitations, which highlight the importance of examining spontaneous pain. The present study describes an automated method for measuring spontaneous pain behaviour in a rat model of neuropathic pain caused by chronic constriction injury (CCI) of sciatic nerve.After CCI surgery, a small magnet was implanted into the operated limb. The rat was placed in a test chamber that was surrounded by wire coil. Limb movements, including lifting/guarding, flinching/shaking, licking and walking in the operated limb, caused changes in the electromagnetic field, including a change in voltage and transformed into a signal via an amplifier.CCI rats consistently showed more frequent limb movement than sham rats. There was no significant correlation between the frequency of spontaneous pain behaviour and the evoked pain symptoms. Treatment with duloxetine (30?mg/kg p.o.) and amitriptyline (30 and 100?mg/kg p.o.) significantly reduced this frequency. Pregabalin at 30?mg/kg p.o. tended to reduce the frequency, and diclofenac up to 10?mg/kg p.o. had no effect.A non-subjective automated method for measuring spontaneous pain behaviour in an animal model of neuropathic pain was established. It is expected that the current system will greatly enhance the analysis of spontaneous pain-related behaviour, which is a predominant symptom in patients with neuropathic pain. The current system may also be valuable in the screening of potential analgesic treatments.
机译：由伤害性刺激引起的戒断反应，即诱发的疼痛测量，通常用作神经性疼痛动物模型的功效终点。但是，它有几个局限性，突出了检查自发性疼痛的重要性。本研究描述了一种在大鼠坐骨神经慢性压迫性损伤（CCI）引起的神经性疼痛模型中测量自发性疼痛行为的自动化方法.CCI手术后，将一块小磁体植入手术的肢体中。将大鼠放置在被线圈包围的测试室中。肢体运动，包括举起/护卫，退缩/摇晃，舔舔和行走在被操作的肢体中，导致电磁场发生变化，包括电压变化并通过放大器转换为信号。假鼠。自发性疼痛行为的频率与诱发的疼痛症状之间无显着相关性。用度洛西汀（30？mg / kg p.o.）和阿米替林（30和100？mg / kg p.o.）治疗显着降低了该频率。普瑞巴林，剂量为30？mg / kg。往往会降低频率，双氯芬酸的最高剂量为10？mg / kg。建立了一种非主观的自动方法来测量神经性疼痛动物模型中的自发性疼痛行为。预期当前的系统将大大增强对自发性疼痛相关行为的分析，这是神经性疼痛患者的主要症状。当前的系统在筛选潜在的止痛药方面可能也很有价值。

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