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首页> 外文期刊>European journal of gastroenterology and hepatology >CD4+CD8+ human small intestinal T cells are decreased in coeliac patients, with CD8 expression downregulated on intra-epithelial T cells in the active disease.
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CD4+CD8+ human small intestinal T cells are decreased in coeliac patients, with CD8 expression downregulated on intra-epithelial T cells in the active disease.

机译：腹腔疾病患者的CD4 + CD8 +人小肠T细胞减少，在活动性疾病中CD8表达在上皮内T细胞上调。

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BACKGROUND/OBJECTIVE: The intestinal lesion of coeliac disease is thought to be initiated and exacerbated by dysregulation of local T-lymphocyte sub-populations. This study examines changes in intestinal T cells from coeliac patients, with a particular focus on CD4CD8 T cells, immunoregulatory cells normally found in relatively high proportions in the small intestine. METHODS: Cells were obtained from duodenal biopsies from active and treated coeliac patients using chelating and reducing agents (epithelial layer) followed by collagenase treatment (lamina propria). Cell yield and viability were assessed and flow cytometric analysis was used to examine CD4CD8 T cells and to quantify CD8 expression. RESULTS: Surprisingly, total T-cell yields in the epithelial layer did not increase in active coeliac disease although enterocyte counts decreased significantly, giving an appearance of infiltration. In active coeliac patients, CD4CD8 T cell percentages were significantly decreased in both the epithelial layerand lamina propria. Levels of CD8 expression by CD4CD8 T cells in the epithelial layer were decreased significantly in patients with active coeliac disease. CD4CD8 T cell proportions did not return to normal in treated coeliac patients whose villous architecture had responded to gluten withdrawal. CONCLUSIONS: No increase of intra-epithelial lymphocytes in the coeliac lesion may require us to reconsider the definition of coeliac disease as an inflammatory condition. Low CD4CD8 populations in treated as well as untreated coeliac patients indicate that these T cells are inherently absent in individuals genetically predisposed to coeliac disease.
机译：背景/目的：腹腔疾病的肠道病变被认为是由于局部T淋巴细胞亚群失调而引发和加重的。这项研究检查了乳糜泻患者肠道T细胞的变化，特别关注CD4CD8 T细胞，这种免疫调节细胞通常在小肠中的比例较高。方法：使用螯合剂和还原剂（上皮层），然后用胶原酶治疗（固有层），从活跃和治疗后的乳糜泻患者的十二指肠活组织检查中获得细胞。评估细胞产量和生存力，并使用流式细胞仪分析检查CD4CD8 T细胞并定量CD8表达。结果：出人意料的是，活动性腹腔疾病的上皮层总T细胞产量没有增加，尽管肠上皮细胞计数显着下降，从而出现了浸润。在活跃的乳糜泻患者中，上皮层和固有层的CD4CD8 T细胞百分比均显着降低。活动性腹腔疾病患者的上皮层CD4CD8 T细胞CD8表达水平显着降低。在绒毛结构对麸质戒断有反应的经治疗的腹腔疾病患者中，CD4CD8 T细胞比例未恢复正常。结论：腹腔病变中上皮内淋巴细胞没有增加可能需要我们重新考虑将腹腔疾病定义为炎性疾病。在治疗和未治疗的乳糜泻患者中，CD4CD8的数量均较低，这表明这些T细胞在遗传上易患乳糜泻的个体中固有地不存在。

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