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首页> 外文期刊>European journal of gynaecological oncology >Histone deacetylase inhibitor trichostatin A modulates cell cycles in A2780 human ovarian cancer cell lines.
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Histone deacetylase inhibitor trichostatin A modulates cell cycles in A2780 human ovarian cancer cell lines.

机译：组蛋白脱乙酰基酶抑制剂曲古抑菌素A调节A2780人卵巢癌细胞系中的细胞周期。

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PURPOSE OF INVESTIGATION: To analyze the effect and mechanism of TSA on cell cycles in human ovarian cancer cells. METHODS: cells were cultured in RPMI 1640 supplemented. Flow cytometry analysis and RT-PCR were used to examine the distribution of cell cycles and the level of P21(WAF/CIPI) mRNA. RESULTS: TSA induced increase of the G2/M phase accompanied by decrease of the S phase and enhanced level of P21(WAF/CIPI) mRNA in a concentration and time-dependent manner in A2780 cells. CONCLUSION: Trichostatin A affects the activity of cyclin-dependent kinase through increased expression of P21(WAF/CIPI) mRNA. TSA causes A2780 cell blockage in the G2/M phase and inhibits cell proliferation of A2780 cells. The minimum level of active TSA is 100 nM and the minimum time is 12 hours. The effect relies on time and concentration.
机译：研究目的：分析TSA对人卵巢癌细胞周期的影响及其机制。方法：在补充的RPMI 1640中培养细胞。流式细胞仪分析和RT-PCR用于检查细胞周期的分布和P21（WAF / CIPI）mRNA的水平。结果：TSA诱导A2 / 780细胞G2 / M期增加，同时S期减少，P21（WAF / CIPI）mRNA水平升高，呈浓度和时间依赖性。结论：曲古他汀A通过增加P21（WAF / CIPI）mRNA的表达影响细胞周期蛋白依赖性激酶的活性。 TSA导致A2780细胞在G2 / M期受阻，并抑制A2780细胞的细胞增殖。活动TSA的最小级别为100 nM，最小时间为12小时。效果取决于时间和注意力。

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