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首页> 外文期刊>European journal of gynaecological oncology >Modulation of microRNA associated with ovarian cancer cells by genistein.
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Modulation of microRNA associated with ovarian cancer cells by genistein.

机译：金雀异黄素对与卵巢癌细胞相关的microRNA的调节。

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PURPOSE: Role of microRNAs in malignancies is well established due their regulatory role in cellular differentiation, proliferation and cell cycle control. Our purpose was to determine miRNA profiles of serially established ovarian cancer cell lines and the effect of genistein treatment. METHODS: Cell lines (UL-3A, UL-3B) were established from one patient during progression of disease. miRNA profiling was performed in untreated and genistein-treated cells. Estrogen receptors (ER) were studied with real-time polymerase chain reaction (RT-PCR) and Western immunoblotting. In vitro migration and invasion assays were utilized. RESULTS: While 108 miRNAs were expressed equally in both cell lines and their genistein-treated counterparts, an additional 53 miRNAs were differentially expressed. Genistein resulted in induction of ERalpha and ERbeta in ovarian cancer cells. A significant reduction in migration and invasion of UL-3A and UL-3B was demonstrated in genistein-treated cells. CONCLUSION: Common and unique miRNA profiles were demonstrated between the two cell lines, some of which were altered by genistein.
机译：目的：由于其在细胞分化，增殖和细胞周期控制中的调节作用，microRNA在恶性肿瘤中的作用已得到充分确立。我们的目的是确定系列建立的卵巢癌细胞系的miRNA图谱以及染料木黄酮的治疗效果。方法：在疾病进展期间从一名患者中建立了细胞系（UL-3A，UL-3B）。在未处理和染料木黄酮处理过的细胞中进行miRNA分析。通过实时聚合酶链反应（RT-PCR）和Western免疫印迹研究雌激素受体（ER）。利用体外迁移和侵袭测定。结果：尽管在两种细胞系中以及在用染料木素处理过的对应物中均表达了108个miRNA，但另外有53个miRNA却差异表达。金雀异黄素导致卵巢癌细胞中ERalpha和ERbeta的诱导。在金雀异黄素处理的细胞中证明了UL-3A和UL-3B迁移和侵袭的显着减少。结论：在两个细胞系之间证实了共同和独特的miRNA谱，其中一些被染料木黄酮改变。

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