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首页> 外文期刊>European journal of gastroenterology and hepatology >Granulocyte colony-stimulating factor improves function of neutrophils from patients with acute liver failure.
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Granulocyte colony-stimulating factor improves function of neutrophils from patients with acute liver failure.

机译：粒细胞集落刺激因子改善急性肝衰竭患者中性粒细胞的功能。

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OBJECTIVE: To evaluate the in vitro effects of granulocyte colony-stimulating factor (G-CSF) on function of neutrophils in acute liver failure (ALF). METHODS: Neutrophil functions (superoxide and hydrogen peroxide production; phagocytosis and killing; complement receptor expression) were determined simultaneously in 23 patients with ALF due to paracetamol overdose and compared with 23 healthy control subjects. RESULTS: Phagocytosis was reduced in neutrophils from ALF patients compared to controls (P< 0.005) and was significantly increased by incubation with 1,000 or 5,000 IU/ ml G-CSF (P< 0.05). This correlated with increased expression of CD11b (r= 0.93) and CD18 (r= 0.98) after incubation with 5,000 IU/ml G-CSF (P< 0.05). Killing was reduced in ALF neutrophils compared to controls (P< 0.005) and was similarly restored by G-CSF (P< 0.005). An increase in killing correlated with increases in production of superoxide (r = 0.96) and hydrogen peroxide (r= 0.97) by ALF neutrophils after incubation with 1,000 and 5,000 IU/ml of G-CSF when formylmethionylleucylphenylalanine (fMLP) was the stimulant. G-CSF at 5,000 IU/ml increased the production of hydrogen peroxide (P< 0.01) when zymosan was the stimulant. CONCLUSIONS: G-CSF improves the neutrophil dysfunction of ALF.
机译：目的：评价粒细胞集落刺激因子（G-CSF）对中性粒细胞在急性肝衰竭（ALF）中的作用的体外作用。方法：同时测定了对乙酰氨基酚过量的23例ALF患者的嗜中性粒细胞功能（超氧化物和过氧化氢的产生，吞噬作用和杀伤力，补体受体的表达），并与23名健康对照者进行了比较。结果：与对照组相比，ALF患者中性粒细胞的吞噬作用降低（P <0.005），并通过与1,000或5,000 IU / ml G-CSF孵育显着增加（P <0.05）。在与5,000 IU / ml G-CSF孵育后，这与CD11b（r = 0.93）和CD18（r = 0.98）表达增加相关（P <0.05）。与对照组相比，ALF中性粒细胞的杀伤力有所降低（P <0.005），而G-CSF的杀伤力也得到了类似的恢复（P <0.005）。当甲酰甲硫酰基亮氨酰苯丙氨酸（fMLP）作为兴奋剂孵育后，与1,000和5,000 IU / ml的G-CSF孵育后，ALF中性粒细胞的杀伤力增加与超氧化物（r = 0.96）和过氧化氢（r = 0.97）的产生增加相关。当酵母聚糖为刺激剂时，5,000 IU / ml的G-CSF增加了过氧化氢的产生（P <0.01）。结论：G-CSF可改善ALF的中性粒细胞功能障碍。

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