...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>European journal of gastroenterology and hepatology >Evaluation of a rapid on-site anti-HCV test as a screening tool for hepatitis C virus infection
【24h】

Evaluation of a rapid on-site anti-HCV test as a screening tool for hepatitis C virus infection

机译：评估快速的现场抗HCV检测作为丙型肝炎病毒感染的筛查工具

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BACKGROUND: In settings such as needle-stick injuries or intravenous drug abuse, immediate knowledge of the anti-hepatitis C virus (HCV) serostatus instead of waiting for the results of a laboratory-based test can be important to guide further medical procedures and appropriate hygienic advises. Thus, a rapid on-site anti-HCV test was evaluated in daily clinical routine and compared with a laboratory-based certified assay. PATIENTS AND METHODS: Ten microliters of serum or EDTA whole blood was analyzed using a chromatographic immunoassay (Toyo anti-HCV test). Results were available on-site 5-15 min after sample centrifugation. The Architect anti-HCV test served as a reference method. RESULTS: Sera of 189 patients were analyzed (without HCV infection: n=105; HCV infection: n=84). The assay was evaluable in 185 cases (98%). The sensitivity and specificity were 99 and 88%, respectively. With EDTA whole blood, the test was evaluable in 47/52 samples (90%). Forty-six of 47 evaluable EDTA tests were concordant with serum results. The one HCV patient with an unevaluable serum test was diagnosed correctly with the EDTA sample. CONCLUSION: The rapid chromatographic anti-HCV immunoassay has limited specificity, which impairs clinical practicability. A positive result warrants re-evaluation with a certified serologic assay.
机译：背景：在针刺伤或静脉吸毒等情况下，立即了解抗丙型肝炎病毒（HCV）血清状况而不是等待实验室测试的结果可能对指导进一步的医疗程序和适当的治疗很重要。卫生建议。因此，在日常临床常规中评估了快速的现场抗HCV检测，并与基于实验室的认证测定法进行了比较。患者和方法：使用色谱免疫测定法（Toyo抗HCV试验）分析了十微升血清或EDTA全血。样品离心5-15分钟后即可获得结果。建筑师的抗HCV测试作为参考方法。结果：分析了189例患者的血清（无HCV感染：n = 105; HCV感染：n = 84）。该方法可评估185例（98％）。敏感性和特异性分别为99％和88％。使用EDTA全血，可在47/52个样本（90％）中对测试进行评估。 47个可评估的EDTA测试中有46个与血清结果一致。用EDTA样品正确诊断出一名血清检验无法评估的HCV患者。结论：快速层析抗HCV免疫分析法特异性有限，损害了临床实用性。阳性结果需要通过认证的血清学分析进行重新评估。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号