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首页> 外文期刊>European Journal of Histochemistry >Immunolocalization of endocan during the endothelial-mesenchymal transition process
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Immunolocalization of endocan during the endothelial-mesenchymal transition process

机译：内皮-间充质转化过程中内皮糖的免疫定位

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Endocan is a dermatan sulfate proteoglycan (DSPG) that has been observed in the cytoplasm of endothelial cells of small and large vessels in lung, kidney, liver, colon, ovary and brain tumors. This DSPG has been implicated in the regulation of cellular activities such as adhesion, migration, and proliferation. Given the important roles played by endocan in such processes, we sought to determine whether this DSPG is present in the chicken embryo aortic wall in embryonic days 12 and 14,when intimai thickening and endothelial transformation are notorious. Immunolabeling of serial paraffin cross-sections revealed endocan immunoreactivity at the endothelium and some mesenchymal cells constituting the intimai thickening but not in the cells arranged in lamellar layers. We also investigated whether endocan was present in monolayers of primary embryonic aortic endothelial cells attached to fibronectin when they were deprived of serum and stimulated with epidermal growth factor. Immunofluorescence determined that in the epidermal growth factor (EGF) condition where separating, detaching, and migrating cells were observed, endocan appeared organized in arrays typical of focal complexes in the leading edge of these cells. In serum-free medium condition in which the endothelial cells displayed a cobblestone appearance, endocan appeared mainly delineating the margin of many cells. This study demonstrates for the first time the presence of endocan during the aortic wall remodeling, and provides evidence that suggests a possible contribution of this DSPG in the endothelial-mesenchymal transition (EndoMT) process.
机译：Endocan是一种硫酸皮肤素蛋白聚糖（DSPG），已在肺，肾，肝，结肠，卵巢和脑肿瘤的小血管和大血管的内皮细胞的细胞质中观察到。该DSPG与细胞活动如粘附，迁移和增殖的调节有关。考虑到内皮糖在此类过程中所起的重要作用，我们试图确定在臭味增稠和内皮转化臭名昭著的第12天和第14天，该DSPG是否存在于鸡胚主动脉壁中。连续石蜡横截面的免疫标记显示内皮细胞在内皮和一些构成间质增厚的间充质细胞中具有免疫反应性，但在层状层中没有。我们还调查了内皮糖在剥夺血清并被表皮生长因子刺激时是否存在于附着于纤连蛋白的原代主动脉主动脉内皮细胞单层中。免疫荧光测定表明，在观察到分离，分离和迁移细胞的表皮生长因子（EGF）条件下，endocan在这些细胞的前缘以典型的焦点复合物阵列出现。在内皮细胞呈现鹅卵石外观的无血清培养基条件下，内皮糖醇的出现主要是描绘了许多细胞的边缘。这项研究首次证明了在主动脉壁重塑过程中内吞的存在，并提供了表明该DSPG在内皮-间充质转化（EndoMT）过程中可能起作用的证据。

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