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首页> 外文期刊>European Journal of Histochemistry >Ultrastructural and immunocytochemical analysis of the XY body in rat and Guinea pig
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Ultrastructural and immunocytochemical analysis of the XY body in rat and Guinea pig

机译：大鼠和豚鼠XY体的超微结构和免疫细胞化学分析

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The formation of the XY body involves the compaction of the extended chromatin to form a mesh of fibrogranular structures. During this process the ribonucleoprotein particles (RNP), which were associated with the chromatin filaments progressively disappear. High resolution immunolocalization indicates that the mature XY body does not contain RNA polymerase II, hnRNPs, or snURNPs. Occasionally chromatin fibrils extend outside of the XY body. These fibrils are frequently associated with nascent RNP fibrils and granules indicating that not all the DNA of the sex chromosomes is transcriptionally inactive. However, transcription is located outside the sex body. The recombination protein Dmc1 is present in nodules associated with the unpaired chromosomal axes of the sex chromosomes located in the XY body. Cytochemical staining methods and in situ hybridization at electron microscopic level show that RNA is present in the unpaired chromosomal axes suggesting that the presence of RNA in the chromosomal axes and in forming synaptonernal complexes is related with the process of final pairing. The sex body and the nucleoli associated with it do not interweave and do not exchange RNA or DNA-containing filaments. These observations indicate that the spatial relation between these structures is just a close proximity, which is, however, very frequent. [References: 54]
机译：XY体的形成涉及扩展染色质的压实以形成纤维状颗粒结构的网孔。在此过程中，与染色质细丝相关的核糖核蛋白颗粒（RNP）逐渐消失。高分辨率免疫定位表明成熟的XY体不包含RNA聚合酶II，hnRNP或snURNP。染色质原纤维有时会延伸到XY体的外部。这些原纤维经常与新生的RNP原纤维和颗粒相关，表明并非所有的性染色体DNA都在转录上是无活性的。但是，转录位于性体外。重组蛋白Dmc1存在于与位于XY体中的性染色体的未配对染色体轴相关的结节中。细胞化学染色方法和电子显微镜下的原位杂交显示，RNA存在于未配对的染色体轴中，这表明RNA在染色体轴中和形成突触神经突复合体的存在与最终配对的过程有关。性体和与其相关的核仁不会交织，也不会交换包含RNA或DNA的细丝。这些观察结果表明，这些结构之间的空间关系非常接近，但是非常频繁。 [参考：54]

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