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Nitrogen addition and mowing affect microbial nitrogen transformations in a C4 grassland in northern China

机译:氮的添加和割草影响中国北方C4草原的微生物氮转化

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Microbial nitrogen (N) transformations play a key role in regulating N cycling in grassland ecosystems. However, there is still little information on how management of semi-arid grassland such as mowing and/or N fertilizer application affects microbial activity and N transformations. In a field experiment in northern China, N was added at a rate of 10 g N m(-2) year(-1) as NH4NO3 to mown and unmown plots (4 x 4 m(2)) and in situ rates of net ammonification (R-amm), nitrification (R-nit) and mineralization (R-min) were followed at monthly intervals for the vegetation growth periods in the years 2006-2009. In addition, we also measured soil microbial biomass carbon (MBC) and nitrogen (MBN), microbial respiration (MR) and peak above-ground biomass in August of each measurement year. Driven by the pronounced inter-annual variability of rainfall, all the properties investigated varied markedly across years. Nevertheless, we were able to demonstrate that over the 4 years N addition significantly stimulated R-nit, R-min and MBN, on average, by 288, 149 and 11.6%, respectively. However, N addition decreased MBC significantly as well as the ratio of MBC:MBN by, on average, 10 and 23%, respectively, whereas an effect of N addition on MR could not be demonstrated. Mowing decreased MBN, MR and qCO(2) significantly by 9, 28 and 24%, respectively, but no effects were found on microbial net N transformation rates and MBC. N addition and mowing interactively affected R-amm and R-min, and MBN, MBC:MBN. In summary, our results indicate a positive effect of N addition but a negative effect of mowing on microbial N transformation in this C4 grassland in northern China.
机译:微生物氮(N)的转化在调节草地生态系统中的氮循环中起关键作用。但是,关于半干旱草原的管理(例如割草和/或施用氮肥)如何影响微生物活性和氮素转化的信息仍然很少。在中国北方的田间试验中,以10 g N m(-2)年(-1)的速率将N作为NH4NO3添加到已割和未割田(4 x 4 m(2))和原位网速中在2006-2009年的植被生长期间,每月进行氨化(R-amm),硝化(R-nit)和矿化(R-min)。此外,我们还在每个测量年的8月测量了土壤微生物生物量碳(MBC)和氮(MBN),微生物呼吸(MR)和地上生物量峰值。在明显的年际降水变化的驱动下,所调查的所有特性在多年中都有显着变化。然而,我们能够证明,在过去的4年中,氮的添加分别显着地分别使R-nit,R-min和MBN分别提高了288、149和11.6%。然而,氮的添加显着降低了MBC以及MBC:MBN的比例,平均分别降低了10%和23%,而无法证明氮的添加对MR的影响。割草分别将MBN,MR和qCO(2)分别降低了9%,28%和24%,但未发现对微生物净氮转化率和MBC的影响。 N的添加和修剪对R-amm和R-min以及MBN,MBC:MBN产生交互影响。总而言之,我们的研究结果表明,在中国北方这个C4草地上,增施氮肥对微生物氮的转化有负面影响。

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