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首页> 外文期刊>European Journal of Histochemistry >Correlation between PARP-1 immunoreactivity and cytomorphological features of parthanatos, a specific cellular death in breast cancer cells
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Correlation between PARP-1 immunoreactivity and cytomorphological features of parthanatos, a specific cellular death in breast cancer cells

机译：PARP-1免疫反应性与parthanatos细胞形态学特征之间的相关性，parthanatos是乳腺癌细胞中的一种特定细胞死亡

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摘要

In parthanatos, a PARP-1 (poly (ADP-ribose) polymerase l)-mediated cell death, dissipation of mitochondrial membrane potential, large-scale DNA fragmentation and chromatin condensation were observed. In contrast to apoptosis, it does not cause apoptotic bodies formation. Although PARP-l-mediated cell death presents loss of membrane integrity similar to necrosis, it does not induce cell swelling. The purpose of the study was to correlate the immunohistochemical parameters of PARP-1 reactivity and the selected cytomorphological features of parthanatos: the lack of apoptotic bodies and the absence of necrosis in breast cancer (BC) specimens. Immunohisto-chemistry for PARP-1 was performed on 83 BC specimens. Correlations between parameters of PARP-1 expression and sub-cellular localisation and the presence of apoptotic bodies and necrosis were evaluated. High expression of PARP-1 (immunoreactive score ≥6) was associated with the lack of apoptotic bodies (P=0.013) and with the absence of necrosis (P=0.002). The presence of apoptotic bodies was correlated with re-distribution of PARP-1 from the nucleus to cytoplasm in BC cells (P=0.029). Additionally, a tendency was observed between necrosis and loss of nuclear PARP-1 expression (P=0.049). Our study suggests that PARP-1 may play a crucial role in induction and regulation of specific type of cellular death called parthanatos.
机译：在parthanatos中，观察到PARP-1（聚（ADP-核糖）聚合酶I）介导的细胞死亡，线粒体膜电位耗散，大规模DNA片段化和染色质浓缩。与凋亡相反，它不会引起凋亡小体的形成。尽管PARP-1介导的细胞死亡与坏死相似，呈现出膜完整性的丧失，但它不会诱导细胞肿胀。该研究的目的是将PARP-1反应性的免疫组织化学参数与parthanatos的所选细胞形态学特征相关联：乳腺癌（BC）标本中缺乏凋亡小体和坏死。 PARP-1的免疫组织化学是在83 BC的标本上进行的。评估了PARP-1表达参数与亚细胞定位以及凋亡小体和坏死的关系。 PARP-1的高表达（免疫反应评分≥6）与凋亡小体的缺乏（P = 0.013）和坏死的缺乏（P = 0.002）有关。凋亡小体的存在与BC细胞中PARP-1从细胞核到细胞质的重新分布有关（P = 0.029）。另外，在坏死和核PARP-1表达丧失之间观察到趋势（P ＝ 0.049）。我们的研究表明，PARP-1可能在诱导和调节称为Parthanatos的特定类型的细胞死亡中起关键作用。

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