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首页> 外文期刊>European Journal of Histochemistry >Dystrophin and dystrophin-associated protein in muscles and nerves from monkey
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Dystrophin and dystrophin-associated protein in muscles and nerves from monkey

机译：猴子肌肉和神经中的肌营养不良蛋白和肌营养不良蛋白相关蛋白

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摘要

Since all organs (i.e. skeletal, cardiac, smooth muscles and sciatic nerve) are never only taken from a single patient, all these tissues were obtained from one cynomolgus monkey, a model closely resembling humans. This work describes an up-to-date reinvestigation of the dystrophin-glycoprotein complex and related molecules in various monkey tissues such those cited above. We used monoclonal and polyclonal antibodies produced in our laboratory, which are directed against dystrophin, utrophin, short-dystrophin products, alpha-dystrobrevin, beta-dystroglycan, alpha-syntrophin, alpha-, beta-, gamma-, delta-, epsilon-sarcoglycan, and sarcospan. For each molecule, we determined their molecular weight and tissue localization. Regardless of the tissue analyzed, at least one dystrophin or utrophin as full-length molecule and one short-dystrophin product or dystrobrevin as proteins belonging to the dystrophin superfamily were found. beta-dystroglycan, beta and delta sarcoglycans were always detected, while other sarcoglycans varied from all to only three components. epsilon sarcoglycan appears to be specific to smooth muscle, which is devoid of a sarcoglycan. Sarcospan is only absent from sciatic nerve structures. Among the different muscles investigated in this study, short dystrophin products are only present in cardiac muscle. All of these findings are summarized in one table, which highlight in one single animal the variability of the dystrophin-glycoprotein complex components in relation with the organ studied. This statement is important because any attempt to estimate protein restoration needs in each study the knowledge of the expected components that should be considered normal. [References: 33]
机译：由于所有器官（即骨骼，心脏，平滑肌和坐骨神经）绝不会仅取自单个患者，因此所有这些组织均取自一只食蟹猴（一种与人类极为相似的模型）。这项工作描述了各种猴组织中的肌营养不良蛋白-糖蛋白复合物和相关分子的最新再研究。我们使用了我们实验室生产的单克隆和多克隆抗体，它们针对肌营养不良蛋白，促肾上腺素，短肌营养不良蛋白产品，α-dystrobrevin，β-dystroglycan，α-syntrophin，α-，β-，γ-，δ-，ε-肌糖蛋白和肌膜肌。对于每个分子，我们确定了它们的分子量和组织定位。无论所分析的组织是什么，都至少发现了一种肌营养不良蛋白或垂体营养素作为全长分子，并且发现了一种肌营养不良蛋白超家族的蛋白质或一种肌营养不良蛋白或短肌营养不良蛋白。总是检测到β-肌营养不良蛋白，β和δ肌糖蛋白，而其他肌糖蛋白的成分从全部到只有三个。 ε肌糖蛋白似乎是平滑肌特有的，而后者没有肌糖蛋白。 Sarcospan仅存在于坐骨神经结构中。在这项研究中研究的不同肌肉中，短肌营养不良蛋白仅存在于心肌中。所有这些发现汇总在一张表中，该表突出了一只动物中的肌营养不良蛋白-糖蛋白复合物成分与所研究器官的变异性。该声明很重要，因为在每次研究中任何试图估计蛋白质修复需求的知识都应该被认为是正常的预期成分。 [参考：33]

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