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首页> 外文期刊>European food research and technology =: Zeitschrift fur Lebensmittel-Untersuchung und -Forschung. A >Development of suitable standards for quantitative determination of persimmon phenol contents in Folin-Ciocalteu and vanillin assays
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Development of suitable standards for quantitative determination of persimmon phenol contents in Folin-Ciocalteu and vanillin assays

机译：制定适用于福林-乔卡尔特和香草醛测定中柿果酚含量定量测定的标准品

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摘要

Use of gallic acid (GA) as a standard in the Folin-Ciocalteu assay leads to an underestimation of phenol content in persimmon extracts so does the widely used catechin (C) in vanillin assay for determining condensed tannins in persimmon extracts. To develop suitable standards for addressing the issue of accuracy, we prepared highly purified persimmon tannin (PT) and its three fractions (PT20, PT40 and PT60), as well as three characteristic structural subunits of PT including epicatechin-3-gallate-(4 beta -> 8, 2 beta -> O -> 7)-epicatechin-3-gallate (A-type ECG dimer), epigallocatechin-3-gallate-(4 beta -> 8, 2 beta -> O -> 7)-epigallocatechin-3-gallate (A-type EGCG dimer) and epicatechin-(4 beta -> 8)-epicatechin (B-type EC dimer). We compared the color yield of commercially available standards including the dimers GA, EGCG, ECG, C and PT and its three fractions in the Folin-Ciocalteu assay and vanillin assay, respectively. Our results suggested that for estimating phenol content in persimmon extracts, EGCG, ECG, C and GA were poor standards in Folin-Ciocalteu assay. We recommended PT40 as the most suitable standard in determining the phenol content of persimmon extracts. In the vanillin assay, A-type EGCG dimer or A-type ECG dimer were superior than the commonly used C as standards, and ECG was also an alternative when A-type EGCG dimer or A-type ECG dimer were not available
机译：在Folin-Ciocalteu分析中使用没食子酸（GA）作为标准品会导致低估柿子提取物中的苯酚含量，因此在香兰素测定中用于确定柿子提取物中浓缩单宁的广泛儿茶素（C）也会被低估。为了制定解决精度问题的合适标准，我们制备了高度纯化的柿子单宁（PT）及其三个馏分（PT20，PT40和PT60）以及PT的三个特征性结构亚基，包括表儿茶素-3-没食子酸酯-（4 beta-> 8、2 beta-> O-> 7）-表儿茶素-3-没食子酸酯（A型ECG二聚体），epigallocatechin-3-gallate-（4 beta-> 8，8，2 beta-> O-> 7） -epigallocatechin-3-gallate（A型EGCG二聚体）和epicatechin-（4 beta-> 8）-epicatechin（B型EC二聚体）。我们分别在Folin-Ciocalteu分析法和香草醛分析法中比较了包括二聚体GA，EGCG，ECG，C和PT及其三部分的市售标准品的颜色产率。我们的结果表明，对于估算柿子提取物中的苯酚含量，EGCG，ECG，C和GA在Folin-Ciocalteu分析中是较差的标准。我们建议将PT40作为确定柿子提取物中酚含量的最合适标准。在香兰素测定中，A型EGCG二聚体或A型ECG二聚体优于常用的C作为标准品，当没有A型EGCG二聚体或A型ECG二聚体时，ECG也是一种替代选择

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