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首页> 外文期刊>European Journal of Medicinal Chemistry: Chimie Therapeutique >Differential expression of 10 sweetpotato peroxidase genes in response to bacterial pathogen, Pectobacterium chrysanthemi
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Differential expression of 10 sweetpotato peroxidase genes in response to bacterial pathogen, Pectobacterium chrysanthemi

机译：10个甘薯过氧化物酶基因对细菌病原体Chrysanthemi的响应的差异表达

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摘要

To understand the function of each peroxidase (POD, EC 1.11.1.7) in terms of biotic stress, changes in POD specific activity and expression of 10 POD genes were investigated in four cultivars of sweetpotato (Ipomoea batatas) after infection with Pectobacterium chrysanthemi. POD specific activity (units mg~(–1) protein) increased from 16 h after inoculation (HAI) in three varieties. POD activities of two cultivars, Shinwhangmi and White Star, reached a maximum level at 24 HAI by about three times compared to mock treatment (MT), and then decreased, whereas those of Zami and Yulmi continuously increased until 36 HAI. Native gel analysis revealed that one POD isoenzyme with a high electrophoretic mobility significantly increased in response to pathogen infection in all cultivars. Additionally, 10 POD genes displayed differential expression patterns upon bacterial infection by northern analysis. Several POD genes such as swpa2, swpa3, swpa4, swpa5, swpb1 were induced upon bacterial infection, but other genes were not. Particularly, swpa4 gene was markedly expressed in response to bacterial infection in four different cultivars, suggesting that this gene has a stress-inducible promoter. These results indicate that some specific POD isoenzymes are involved in defense in relation to pathogenesis of P. chrysanthemi in sweetpotato plants.
机译：为了了解每种过氧化物酶（POD，EC 1.11.1.7）在生物胁迫方面的功能，研究了在四个感染了金边杆菌的甘薯（番薯（Ipomoea batatas））中POD比活性的变化和10种POD基因的表达。在三个品种中，从接种后（HAI）16 h开始，POD的比活性（单位mg〜（–1）蛋白）增加。与模拟处理（MT）相比，两个品种Shinwhangmi和White Star的POD活性在24 HAI达到最高水平约三倍，然后降低，而Zami和Yulmi的POD活性持续增加直到36 HAI。天然凝胶分析表明，一种具有高电泳迁移率的POD同工酶在所有品种中均响应病原体感染而显着增加。另外，通过Northern分析，细菌感染后10个POD基因显示出差异表达模式。细菌感染后诱导了几种POD基因，例如swpa2，swpa3，swpa4，swpa5，swbb1，但没有其他基因。特别是，swpa4基因在四个不同的品种中对细菌感染有明显的表达，表明该基因具有应激诱导型启动子。这些结果表明，在甘薯植株中，一些特定的POD同工酶参与了与菊花青霉的发病机理有关的防御。

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