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首页> 外文期刊>European Journal of Medicinal Chemistry: Chimie Therapeutique >Isolation and functional assessment of a tomato proteinase inhibitor II gene
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Isolation and functional assessment of a tomato proteinase inhibitor II gene

机译：番茄蛋白酶抑制剂II基因的分离和功能评估

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摘要

A genomic clone encoding a serine proteinase inhibitor II, designated as TPI-2, was isolated from tomato (Lycopersicon esculentum Mill.) seedling. It consisted of a 990 bp upstream regulatory region and a 680 bp transcription region containing an intron. As shown by northern hybridization, mechanical injury activated its expression in roots, stems and leaves, and so did exogenous hormones jasmonic acid (JA) and α-Linolenic acid (LA), though abscisic acid (ABA) and NaCl failed to induce its expression. Salicylic acid (SA) was found to inhibit the inducing effect of LA but not those of mechanical injury and JA. As demonstrated experimentally, TPI-2 could be expressed effectively in tobacco cells and the protein products showed insecticidal activity.
机译：从番茄（Lycopersicon esculentum Mill。）幼苗中分离出编码丝氨酸蛋白酶抑制剂II的基因组克隆，命名为TPI-2。它由一个990 bp的上游调节区和一个680 bp的含有内含子的转录区组成。如北部杂交所示，机械损伤激活了其在根，茎和叶中的表达，外源激素茉莉酸（JA）和α-亚麻酸（LA）也被激活，尽管脱落酸（ABA）和NaCl未能诱导其表达。水杨酸（SA）抑制LA的诱导作用，但不抑制机械损伤和JA的诱导作用。如实验所证明的，TPI-2可以在烟草细胞中有效表达，并且蛋白质产物显示出杀虫活性。

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