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首页> 外文期刊>European Journal of Cell Biology: Journal of Deutsche Gesellschaft fur Elektronenmikroskopie: Journal of Deutsche Gesellschaft fur Zellbiologie >IQGAP and mitotic exit network (MEN) proteins are required for cytokinesis and re-polarization of the actin cytoskeleton in the budding yeast, Saccharomyces cerevisiae
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IQGAP and mitotic exit network (MEN) proteins are required for cytokinesis and re-polarization of the actin cytoskeleton in the budding yeast, Saccharomyces cerevisiae

机译：IQGAP和有丝分裂出口网络（MEN）蛋白是芽胞酵母酿酒酵母中胞质分裂和肌动蛋白细胞骨架重新极化所必需的

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摘要

In budding yeast the final stages of the cell division cycle, cytokinesis and cell separation, are distinct events that require to be coupled, both together and with mitotic exit. Here we demonstrate that mutations in genes of the mitotic exit network (MEN) prevent cell separation and are synthetically lethal in combination with both cytokinesis and septation defective mutations. Analysis of the synthetic lethal phenotypes reveals that Iqg1p functions in combination with the MEN components, Tem1p, Cdc15p Dbf20p and Dbf2p to govern the re-polarization of the actin cytoskeleton to either side of the bud neck. In addition phosphorylation of the conserved PCH protein, Hof1p, is dependent upon these activities and requires actin ring assembly. Recruitment of Dbf2p to the bud neck is dependent upon actin ring assembly and correlates with Hof1p phosphorylation. Failure to phosphorylate Hof1p results in the increased stability of the protein and its persistence at the bud neck. These data establish a mechanistic dependency of cell separation upon an intermediate step requiring actomyosin ring assembly.
机译：在发芽酵母中，细胞分裂周期的最后阶段，胞质分裂和细胞分离是需要结合在一起以及与有丝分裂退出结合的不同事件。在这里，我们证明了有丝分裂出口网络（MEN）的基因中的突变可防止细胞分离，并且与胞质分裂和分隔缺陷突变共同导致致命。对合成致死表型的分析表明，Iqg1p与MEN组分Tem1p，Cdc15p Dbf20p和Dbf2p结合起作用，控制肌动蛋白细胞骨架向芽颈两侧重新极化。此外，保守的PCH蛋白Hof1p的磷酸化依赖于这些活性，并需要肌动蛋白环组装。 Dbf2p向芽颈的募集取决于肌动蛋白环的组装，并与Hof1p磷酸化相关。未能磷酸化Hof1p会导致该蛋白质的稳定性增加，并且在芽颈处持续存在。这些数据建立了细胞分离对需要放线菌素环组装的中间步骤的机械依赖性。

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