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首页> 外文期刊>Immunology: An Official Journal of the British Society for Immunology >Photodynamic therapy with recombinant adenovirus AdmIL-12 enhances anti-tumour therapy efficacy in human papillomavirus 16 (E6/E7) infected tumour model.
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Photodynamic therapy with recombinant adenovirus AdmIL-12 enhances anti-tumour therapy efficacy in human papillomavirus 16 (E6/E7) infected tumour model.

机译：重组腺病毒AdmIL-12的光动力疗法可增强人乳头瘤病毒16（E6 / E7）感染的肿瘤模型的抗肿瘤治疗功效。

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Immunotherapy with photodynamic therapy (PDT) offers great promise as a new alternative for cancer treatment; however, its use remains experimental. Here we investigated the utility of adenoviral delivery of interleukin-12 (AdmIL-12) as an adjuvant for PDT in mouse tumour challenge model. PDT was performed by irradiating Radachlorin in C57BL/6 mice transplanted with TC-1 cells. PDT plus AdmIL-12 treatment for tumour suppression as well as specific immune responses were evaluated with the following tests: in vitro and in vivo tumour growth inhibition, interferon-gamma (IFN-gamma) and tumour necrosis factor-alpha (TNF-alpha) assay, and cytotoxic T lymphocyte (CTL) assay. Direct intratumoral injection of AdmIL-12 resulted in a significant suppression of tumour growth compared to the control group. Treatment of PDT along with AdmIL-12 further enhanced antitumour effects significantly higher than either AdmIL-12 or PDT alone. This combined treatment resulted in complete regression of 9-mm sized tumour in every animal. We also evaluated immune responses induced by these treatments. Combined treatment significantly increased the production level of IFN-gamma and TNF-alpha compared with that by AdmIL-12 or PDT alone. PDT plus AdmIL-12 enhanced antitumour immunity through increased expansion of the CTL subset mediated by CD8+ T cells. Taken together, these results indicate that the high anti-cancer activity of PDT with AdmIL-12 is a powerful tool against cancer therapy and is a promising subject for further investigation.
机译：带有光动力疗法（PDT）的免疫疗法有望成为癌症治疗的新选择。但是，其使用仍处于实验阶段。在这里，我们研究了白细胞介素12（AdmIL-12）的腺病毒作为小鼠肿瘤激发模型中PDT的佐剂的效用。通过在移植了TC-1细胞的C57BL / 6小鼠中照射Radachlorin进行PDT。使用以下测试评估了PDT加AdmIL-12治疗肿瘤的抑制作用以及特异性免疫应答：体内和体外肿瘤生长抑制，干扰素-γ（IFN-γ）和肿瘤坏死因子-α（TNF-α）检测和细胞毒性T淋巴细胞（CTL）检测。与对照组相比，直接在肿瘤内注射AdmIL-12可显着抑制肿瘤生长。与AdmIL-12或PDT相比，PDT与AdmIL-12的治疗进一步增强了抗肿瘤作用。这种联合治疗导致每只动物9毫米大小的肿瘤完全消退。我们还评估了这些疗法诱导的免疫反应。与单独使用AdmIL-12或PDT相比，联合治疗显着提高了IFN-γ和TNF-α的产生水平。 PDT加AdmIL-12通过增加CD8 + T细胞介导的CTL亚群的扩增来增强抗肿瘤免疫力。综上所述，这些结果表明，带有AdmIL-12的PDT的高抗癌活性是抗癌治疗的有力工具，并且是有希望进一步研究的课题。

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