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首页> 外文期刊>Immunology: An Official Journal of the British Society for Immunology >Interleukin-4 can induce interleukin-4 production in dendritic cells.
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Interleukin-4 can induce interleukin-4 production in dendritic cells.

机译：IL-4可以诱导树突状细胞中IL-4的产生。

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The presence of interleukin-4 (IL-4) during the generation of dendritic cells (DC) from precursor cells results in measurable increases of IL-12 in supernatants but IL-4 secretion has not been reported. However, DC have IL-4 receptors and are able to make IL-4. We therefore sought evidence for autocrine effects of IL-4 on DC. IL-4 gene expression was low in DC generated from bone-marrow stem cells in the presence of granulocyte-macrophage colony-stimulating factor but was up-regulated by exposure of the developing DC to IL-4. Exposure to IL-4 also induced intracellular IL-4 production in DC. The intracellular IL-4 induced in the presence of IL-4 was increased following further DC maturation with tumour necrosis factor-alpha. By contrast, in supernatants of DC, IL-4 was rarely detected and only at late culture periods. However, after exposure of DC to IL-4, cell-bound IL-4 was detected transiently, which suggested binding and internalization of the cytokine. Binding via IL-4 receptor-alpha was indicatedfrom phosphorylation of the signal transducer and activator of transcription (STAT) protein 6, which is known to mediate IL-4 function. Cytokine persisting within the supernatants of the cells may therefore be unrepresentative of the actual production and function of IL-4 in the cells; IL-4 may be produced in DC in response to exposure to IL-4 but may then be lost from the supernatants during cell binding and activation of the cells.
机译：从前体细胞生成树突状细胞（DC）期间，白介素4（IL-4）的存在导致上清液中IL-12的可测量增加，但尚未报道IL-4分泌。但是，DC具有IL-4受体并能够产生IL-4。因此，我们寻求IL-4对DC的自分泌作用的证据。在存在粒细胞-巨噬细胞集落刺激因子的情况下，骨髓干细胞产生的DC中IL-4基因表达较低，但通过将发育中的DC暴露于IL-4则上调IL-4基因表达。暴露于IL-4也会诱导DC中细胞内IL-4的产生。用肿瘤坏死因子-α进一步DC成熟后，在IL-4存在下诱导的细胞内IL-4增加。相比之下，在DC的上清液中很少检测到IL-4，仅在培养后期才检测到。然而，将DC暴露于IL-4后，瞬时检测到细胞结合的IL-4，这提示细胞因子的结合和内在化。信号介导子和转录激活子（STAT）蛋白6的磷酸化表明了通过IL-4受体-α的结合，该蛋白已知介导IL-4功能。因此，存在于细胞上清液中的细胞因子可能无法代表细胞中IL-4的实际产生和功能。 IL-4可能会因暴露于IL-4而在DC中产生，但随后在细胞结合和细胞活化过程中会从上清液中丢失。

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