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首页> 外文期刊>Immunology: An Official Journal of the British Society for Immunology >A vaccine formulated with the major outer membrane protein can protect C3H/HeN, a highly susceptible strain of mice, from a Chlamydia muridarum genital challenge
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A vaccine formulated with the major outer membrane protein can protect C3H/HeN, a highly susceptible strain of mice, from a Chlamydia muridarum genital challenge

机译：用主要的外膜蛋白配制的疫苗可以保护C3H / HeN（一种高度易感的小鼠品系）免于感染衣原体生殖器

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C3H/HeN female mice were vaccinated with native Chlamydia muridarum major outer membrane protein (MOMP), using Montanide+CpG or Alum+CpG as adjuvants. Negative control groups were immunized with ovalbumin (OVA) and the same adjuvants. As positive control, mice were inoculated intranasally with live Chlamydia. Mice were challenged in the ovarian bursa with 10(5)C.muridarum inclusion forming units. Six weeks after the genital challenge the animals were caged with male mice and monitored for pregnancy. Mice vaccinated with MOMP+Montanide+CpG developed high levels of C.muridarum-specific antibodies, with a high IgG2a/IgG1 ratio and neutralizing titres. Animals immunized using Alum+CpG had low antibody levels. Cellular immune responses were significantly higher in mice vaccinated with MOMP and Montanide+CpG, but not with Alum+CpG, when compared with negative controls. Following the genital challenge, only 20% (4/20) of mice vaccinated with MOMP+CpG+Montanide had positive vaginal cultures whereas 100% (9/9) of mice immunized with MOMP+CpG+Alum had positive cultures. Of the positive control animals inoculated with live Chlamydia only 15% (3/20) had positive vaginal cultures. In contrast, 100% (20/20) of mice immunized with OVA+CpG+Montanide, or minimal essential medium, had positive cultures. Following mating, 80% (16/20) of mice vaccinated with MOMP+CpG+Montanide, and 85% (17/20) of animals inoculated intranasally with live C.muridarum carried embryos in both uterine horns. No protection against infertility was observed in mice immunized with MOMP and CpG+Alum or OVA. In conclusion, this is the first time that a subunit vaccine has been shown to elicit a protective immune response in the highly susceptible C3H/HeN strain of mice against an upper genital challenge.
机译：使用Montanide + CpG或Alum + CpG作为佐剂，向C3H / HeN雌性小鼠接种天然的衣原体衣原体主要外膜蛋白（MOMP）。阴性对照组用卵清蛋白（OVA）和相同的佐剂免疫。作为阳性对照，用活衣原体鼻内接种小鼠。用10（5）C.muridarum包涵体形成单位在卵巢法氏囊中攻击小鼠。生殖器攻击后六周，将动物圈养在雄性小鼠中并监测怀孕。接种了MOMP + Montanide + CpG的小鼠产生了高水平的衣原体特异性抗体，并具有较高的IgG2a / IgG1比和中和效价。使用Alum + CpG免疫的动物抗体水平较低。与阴性对照相比，在接种了MOMP和Montanide + CpG的小鼠中，细胞免疫应答显着更高，但没有接种Alum + CpG。进行生殖器攻击后，只有20％（4/20）的MOMP + CpG + Montanide疫苗接种的小鼠具有阳性阴道培养物，而100％（9/9）的MOMP + CpG + Alum免疫的小鼠具有阳性培养物。在接种活衣原体的阳性对照动物中，只有15％（3/20）的阴道培养物阳性。相反，用OVA + CpG + Montanide或基本必需培养基免疫的100％（20/20）小鼠培养阳性。交配后，80％（16/20）的小鼠接受了MOMP + CpG + Montanide的疫苗接种，而85％（17/20）的动物经鼻内接种了活的乌门氏衣原体，在两个子宫角中均带有胚胎。在用MOMP和CpG +明矾或OVA免疫的小鼠中未观察到针对不育的保护作用。总之，这是首次证明亚单位疫苗可在高度敏感的C3H / HeN小鼠品系中引发针对上生殖器攻击的保护性免疫反应。

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