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首页> 外文期刊>Immunology: An Official Journal of the British Society for Immunology >Induction of autoimmunity by immunization with hapten-modified hen egg lysozyme in hen egg lysozyme-transgenic mice.
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Induction of autoimmunity by immunization with hapten-modified hen egg lysozyme in hen egg lysozyme-transgenic mice.

机译：通过半抗原修饰的鸡蛋溶菌酶在鸡鸡蛋溶菌酶转基因小鼠中的免疫诱导自身免疫。

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摘要

To understand the mechanism of autoimmunity induction, hen egg lysozyme (HEL)-transgenic (Tg) C57BL/6 (B6) mice were immunized with HEL or phosphorylcholine-conjugated HEL (PC-HEL). Repeated immunization of HEL-Tg mice with native HEL failed to induce the antibody response against HEL. However, immunization with PC-HEL generated a significant anti-HEL antibody response. Immunization of the Tg mice with dominant (HEL(74-88)) or cryptic (HEL(47-61)) T-cell epitope peptide stimulated the corresponding T-cell response and similarly yielded the anti-HEL antibody response. Predominance of immunoglobulin G1 (IgG1) anti-HEL antibody response in the HEL-Tg mice and preferential IL-4 production by HEL-specific T cells suggested the dependency of the antibody response to the presence of T helper 2. HEL-Tg mice received HEL-primed B6 T cells, but not HEL-primed Tg T cells, were able to generate anti-HEL antibody response following PC-HEL immunization. The pattern and the level of epitope peptides generated by splenic antigen-presenting cells indicated that PC-HEL results in much more efficient processing as compared to HEL. These results strongly suggest that the enhancement of antigen processing by hapten (PC) conjugation to the antigen facilitates more efficient stimulation of T cells reactive to self antigen, HEL in HEL-Tg mice resulting in the production of anti-self HEL antibody.
机译：为了了解自身免疫诱导的机制，将鸡蛋溶菌酶（HEL）-转基因（Tg）C57BL / 6（B6）小鼠用HEL或结合磷酸胆碱的HEL（PC-HEL）进行免疫。用天然HEL重复接种HEL-Tg小鼠未能诱导针对HEL的抗体应答。但是，用PC-HEL免疫会产生明显的抗HEL抗体反应。用显性（HEL（74-88））或隐性（HEL（47-61））T细胞表位肽免疫Tg小鼠会刺激相应的T细胞反应，并类似地产生抗HEL抗体反应。 HEL-Tg小鼠中免疫球蛋白G1（IgG1）抗HEL抗体应答占优势，HEL特异性T细胞优先产生IL-4，这表明抗体应答对T辅助细胞2的依赖性。在PC-HEL免疫后，HEL引发的B6 T细胞而非HEL引发的Tg T细胞能够产生抗HEL抗体反应。脾抗原呈递细胞产生的抗原决定簇肽的模式和水平表明，与HEL相比，PC-HEL的处理效率更高。这些结果强烈表明，通过半抗原（PC）与抗原的缀合来增强抗原加工，有助于更有效地刺激HEL-Tg小鼠中与自身抗原HEL反应的T细胞，从而产生抗自身HEL抗体。

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