• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Immunology: An Official Journal of the British Society for Immunology >Function and expression of CD1d and invariant natural killer T-cell receptor in the cotton rat (Sigmodon hispidus)
【24h】

Function and expression of CD1d and invariant natural killer T-cell receptor in the cotton rat (Sigmodon hispidus)

机译：棉鼠（Sigmodon hispidus）CD1d和不变的自然杀伤性T细胞受体的功能和表达

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The cotton rat (Sigmodon hispidus) belongs to the rodent family of Cricetidae and provides a powerful model to study the pathogenesis of human respiratory viruses and measles virus. Recent studies in other rodent models have suggested a role for invariant natural killer T (iNKT) cells in antiviral immunity and vaccination against respiratory virus infections. Using new experimental tools, we provide the first evidence for a functional CD1d cell molecule (crCD1d) and iNKT T-cell receptor in cotton rats. The crCD1d cDNA sequence was identified and crCD1d transductants showed that monoclonal antibody WTH-2 stains crCD1d as efficiently as mouse or rat CD1d. The expression of crCD1d was clearly weaker for thymocytes and B cells, and higher for T cells, which is different to what is found in murine species. The antigen-presenting capacity of crCD1d was demonstrated with crCD1d-immunoglobulin dimers loaded with the glycolipid PBS57, which bound iNKT T-cell receptors. Evidence for functional cotton rat iNKT cells was provided by detection of interferon-c and interleukin-4 in cultures of splenocytes stimulated with PBS57 and a-galactosylceramide and by specific staining of about 0.2% of splenocytes with PBS57-loaded crCD1d dimers. Canonical AV14/AJ18 rearrangements were identified and found to contain multiple members of the AV14 (AV11) family. One of them was expressed and found to bind CD1d dimers. In summary, these data provide the first evidence for functional CD1d molecules and iNKT T-cell receptors in cotton rats and provide the tools to analyse them both in the cotton rat model of infectious diseases.
机译：棉鼠（Sigmodon hispidus）属于C科的啮齿动物科，为研究人类呼吸道病毒和麻疹病毒的发病机理提供了有力的模型。在其他啮齿动物模型中的最新研究表明，不变的自然杀伤T（iNKT）细胞在抗病毒免疫和接种呼吸道病毒感染的疫苗中发挥作用。使用新的实验工具，我们提供了棉鼠中功能性CD1d细胞分子（crCD1d）和iNKT T细胞受体的第一个证据。鉴定了crCD1d cDNA序列，crCD1d转导物显示单克隆抗体WTH-2对crCD1d的染色与小鼠或大鼠CD1d一样有效。 crCD1d的表达对于胸腺细胞和B细胞明显较弱，对于T细胞则较高，这与在鼠类中发现的不同。用装载糖脂PBS57的crCD1d-免疫球蛋白二聚体证明了crCD1d的抗原呈递能力，该二聚体结合了iNKT T细胞受体。通过检测用PBS57和α-半乳糖苷神经酰胺刺激的脾细胞培养物中干扰素-c和白介素-4的检测，以及用PBS57负载的crCD1d二聚体对约0.2％的脾细胞进行特异性染色，可以提供功能性棉鼠iNKT细胞的证据。确定了规范的AV14 / AJ18重排，发现其中包含AV14（AV11）系列的多个成员。表达其中之一并发现其结合CD1d二聚体。总之，这些数据为棉鼠的功能性CD1d分子和iNKT T细胞受体提供了第一个证据，并提供了在棉鼠传染病模型中对它们进行分析的工具。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号