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首页> 外文期刊>Immunology: An Official Journal of the British Society for Immunology >Soluble CD276 (B7-H3) is released from monocytes, dendritic cells and activated T cells and is detectable in normal human serum.
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Soluble CD276 (B7-H3) is released from monocytes, dendritic cells and activated T cells and is detectable in normal human serum.

机译：可溶性CD276（B7-H3）从单核细胞，树突状细胞和活化的T细胞释放出来，并且在正常人血清中可检测到。

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Expression of membrane CD276 (mB7-H3) has been reported on dendritic cells (DCs), monocytes, activated T cells, and various carcinoma cells. However, reports concerning its in vivo function have been inconsistent. Moreover, whether there is a soluble form of this protein is not known. In this study, using a sensitive dual monoclonal antibody sandwich enzyme-linked immunosorbent assay (ELISA) to detect the soluble form of B7-H3 (sB7-H3), we demonstrated the release of sB7-H3 by monocytes, DCs, activated T cells, and various mB7-H3+ but not mB7-H3- carcinoma cells. Release from cells was blocked by addition of a matrix metalloproteinase inhibitor (MMPI), which concomitantly caused the accumulation of B7-H3 on the cell surface. To determine the level of circulating sB7-H3, more than 200 serum samples were included in the study. The results indicated that sB7-H3 was present at high levels in all serum samples. Western blotting of sB7-H3 from cell culture supernatants or sera of healthy donors indicated that the molecular size was approximately 16 kDa. Soluble B7-H3 was able to bind to the B7-H3 receptor (B7-H3R) on activated T cells, which showed that sB7-H3 is a functionally active form. These results indicate that release of sB7-H3 from the cell surface is mediated by a matrix metalloproteinase and probably regulates B7-H3R/B7-H3 interactions in vivo. Cleavage of sB7-H3 to an active soluble form would alter both proximal and distal cellular responses.
机译：膜CD276（mB7-H3）的表达已报道在树突状细胞（DC），单核细胞，活化的T细胞和各种癌细胞上。但是，有关其体内功能的报道并不一致。而且，尚不知道该蛋白是否存在可溶形式。在这项研究中，使用敏感的双单克隆抗体夹心酶联免疫吸附测定（ELISA）检测B7-H3（sB7-H3）的可溶性形式，我们证明了单核细胞，DC，活化T细胞释放sB7-H3和各种mB7-H3 +，但不包括mB7-H3-癌细胞。通过添加基质金属蛋白酶抑制剂（MMPI）阻止从细胞中释放，该抑制剂同时导致B7-H3在细胞表面积聚。为了确定循环中sB7-H3的水平，研究中包括了200多个血清样品。结果表明，所有血清样品中sB7-H3含量高。来自健康供体的细胞培养上清液或血清的sB7-H3的Western印迹表明，分子大小约为16 kDa。可溶性B7-H3能够与活化T细胞上的B7-H3受体（B7-H3R）结合，这表明sB7-H3是一种功能活性形式。这些结果表明，sB7-H3从细胞表面的释放是由基质金属蛋白酶介导的，并且可能在体内调节B7-H3R / B7-H3的相互作用。将sB7-H3裂解为活性可溶形式会改变近端和远端细胞反应。

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