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首页> 外文期刊>European Journal of Phycology >Taxonomy and production of paralytic shellfish toxins by thefreshwater cyanobacterium Aphanizomenon gracile LMECYA40
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Taxonomy and production of paralytic shellfish toxins by thefreshwater cyanobacterium Aphanizomenon gracile LMECYA40

机译：淡水蓝藻Aphanizomenon gracile LMECYA40的分类学和麻痹性贝类毒素的产生

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摘要

Detection of Paralytic Shellfish Toxins (PST) in an algal bloom in a Portuguese drinking water reservoir (Lake Crato) was followed by isolation of a strain of Aphanizomenon gracile. The strain, coded as LMECYA40, was cultured and identified by combining a morphological study with 16S rRNA gene sequencing. The toxin profile of this isolate, as revealed by HPLC-FLD analysis, was similar to that of other Aphanizomenon strains (e.g. NH-5) isolated from North America, consisting of two PST analogues: neoSTX (0.27 fmol/cell) and STX (0.05 fmol/cell). Based on these toxin levels, we estimated that a culture with a cell density of 10 super(7) cells/ml should contain approximately 910 mu g of STX equivalents per liter. Small volumes of water containing PST concentrations similar to those estimated from the cultured A. gracile would therefore contain PST amounts comparable to those used as limit enforcement for harvesting and consumption of shellfish in marine environments (80 mu g of STX equivalents per 100 g of shellfish).
机译：在葡萄牙饮用水水库（克拉托湖）的藻华中检测到麻痹性贝类毒素（PST），然后分离出一种Aphanizomenon gracile菌株。通过将形态学研究与16S rRNA基因测序相结合，对菌株LMECYA40进行了培养和鉴定。 HPLC-FLD分析显示，该菌株的毒素谱与从北美分离出的其他Aphanizomenon菌株（例如NH-5）相似，由两个PST类似物组成：neoSTX（0.27 fmol / cell）和STX（ 0.05 fmol /细胞）。基于这些毒素水平，我们估计细胞密度为10 super（7）细胞/ ml的培养物每升应包含约910μgSTX当量。因此，少量的PST浓度与从养殖的拟南芥中估计的浓度相似的PST水中的PST量可与海洋环境中捕捞和食用贝类的限量执行所使用的PST量相当（每100克贝类中80克STX当量） ）。

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