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Occurrence of lucerne seed-borne Enterobacter cloacae sprout decay in Gansu Province of China

机译:甘肃省卢塞恩种子传播的阴沟肠杆菌芽苗的发生

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摘要

A previously unreported sprout decay disease of lucerne affecting as many as 15% of sprouts was observed in Northwest China (Gansu province) in May of 2011. Bacteria were isolated and a range of identification methods undertaken including bacteriological tests, carbon source utilization profiling (Biolog) and 16S rDNA sequence analysis. The bacterial isolates (YZ-DS 1, 2, and 3) were finally identified as Enterobacter cloacae. Lucerne seed inoculations revealed that E. cloacae isolated from infected sprouts produced a yellowing and rot similar to that observed on the original symptomatic sprouts, thus identifying E. cloacae as the causal agent of the outbreak. To our knowledge, this is first report of E. cloacae causing sprout decay of lucerne worldwide.
机译:2011年5月,在中国西北地区(甘肃省)发现了一种以前从未报道过的影响到15%芽苗的卢塞恩芽苗病。分离了细菌,并进行了一系列鉴定方法,包括细菌学测试,碳源利用谱分析(Biolog )和16S rDNA序列分析。最终分离出细菌分离株(YZ-DS 1、2和3)为阴沟肠杆菌。卢塞恩种子接种显示,从感染的新芽中分离出的阴沟肠杆菌产生黄变和腐烂,类似于在原始症状豆芽上观察到的,因此将阴沟肠杆菌鉴定为爆发的病原体。据我们所知,这是首次报道阴沟肠杆菌引起全世界卢塞恩发芽腐烂。

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