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首页> 外文期刊>Experimental and therapeutic medicine >Malignant behaviorial characteristics of CD133(+/-) glioblastoma cells from a Northern Chinese population
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Malignant behaviorial characteristics of CD133(+/-) glioblastoma cells from a Northern Chinese population

机译：中国北方人群CD133（+/-）胶质母细胞瘤细胞的恶性行为特征

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Following emergence of the tumor stem cell theory, the increasing number of related studies demonstrates the theory's growing importance in cancer research and its potential for clinical applications. Few studies have addressed the in vitro or in vivo properties of glioma stem cells from a Han Chinese population. In the present study, surgically obtained glioblastoma tissue was classified into two subtypes, CD133(+) and CD133(-). The hierarchy, invasiveness, growth tolerance under low nutrient conditions and colony forming abilities of the tissue samples were analyzed. Additionally, the characteristics of tumor cells transplanted subcutaneously or re-transplanted into nude mice were observed. The results demonstrated that CD133(+) glioblastoma cells derived from Han Chinese glioma specimens were more prone to primitive cell differentiation and more invasive than CD133(-) glioblastoma cells, leading to increased tumor malignancy compared with CD133(-) cells. The tumor formation rates of CD133(+) and CD133(-) cells in mice were 26/30 and 2/30, respectively. A comparison of tumor subtypes demonstrated that CD133(+) glioblastoma cells had a lower incidence of cell apoptosis in the tumor tissue and higher protein expression levels of Oct4, Sox2, PCNA, EGFR, Ang2, MMP2 and MMP9 compared with CD133(-) cells. Flow cytometry revealed that in the CD133(+) and CD133(-) glioblastoma cell-induced tumors, the percentage of CD133(+) cells was 2.47 +/- 0.67 and 0.44 +/- 0.14%, respectively. The tumor formation rates following the re-transplantation of CD133(+) or CD133(-) tumors into nude mice were 10/10 and 4/10, respectively. These findings suggest that the CD133(+) glioblastoma cell subpopulation has a stronger malignant cell phenotype than the CD133(-) subpopulation and that its recurrence rate is increased compared with the primitive tumorigenic rate following in vivo transplantation.
机译：随着肿瘤干细胞理论的出现，越来越多的相关研究证明了该理论在癌症研究中的重要性及其在临床上的潜力。很少有研究涉及汉族人群神经胶质瘤干细胞的体外或体内特性。在本研究中，手术获得的胶质母细胞瘤组织分为两种亚型，CD133（+）和CD133（-）。分析了低营养条件下的层次，侵袭性，生长耐受性以及组织样本的菌落形成能力。另外，观察到了皮下移植或再移植到裸鼠中的肿瘤细胞的特征。结果表明，汉族神经胶质瘤标本中的CD133（+）胶质母细胞瘤细胞比CD133（-）胶质母细胞瘤细胞更容易发生原始细胞分化和侵袭，与CD133（-）细胞相比，导致恶性肿瘤增加。小鼠中CD133（+）和CD133（-）细胞的肿瘤形成率分别为26/30和2/30。肿瘤亚型的比较表明，与CD133（-）细胞相比，CD133（+）胶质母细胞瘤细胞在肿瘤组织中具有较低的细胞凋亡发生率，Oct4，Sox2，PCNA，EGFR，Ang2，MMP2和MMP9的蛋白表达水平较高。 。流式细胞仪显示，在CD133（+）和CD133（-）胶质母细胞瘤细胞诱导的肿瘤中，CD133（+）细胞的百分比分别为2.47 +/- 0.67和0.44 +/- 0.14％。将CD133（+）或CD133（-）肿瘤重新移植到裸鼠中后的肿瘤形成率分别为10/10和4/10。这些发现表明，CD133（+）胶质母细胞瘤细胞亚群比CD133（-）亚群具有更强的恶性细胞表型，并且与体内移植后的原始致瘤率相比，其复发率有所提高。

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