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首页> 外文期刊>Experimental Neurology >Olfactory ensheathing cells, olfactory nerve fibroblasts and biomatrices to promote long-distance axon regrowth and functional recovery in the dorsally hemisected adult rat spinal cord.
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Olfactory ensheathing cells, olfactory nerve fibroblasts and biomatrices to promote long-distance axon regrowth and functional recovery in the dorsally hemisected adult rat spinal cord.

机译：嗅鞘细胞，嗅神经成纤维细胞和生物基质可促进背侧成年大鼠脊髓的长轴突再生和功能恢复。

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摘要

Cellular transplantation, including olfactory ensheathing cells (OEC) and olfactory nerve fibroblasts (ONF), after experimental spinal cord injury in the rat has previously resulted in regrowth of severed corticospinal (CS) axons across small lesion gaps and partial functional recovery. In order to stimulate CS axon regrowth across large lesion gaps, we used a multifactorial transplantation strategy to create an OEC/ONF continuum in spinal cords with a 2-mm-long dorsal hemisection lesion gap. This strategy involved the use of aligned OEC/ONF-poly(D,L)-lactide biomatrix bridges within the lesion gap and OEC/ONF injections at 1 mm rostral and caudal to the lesion gap. In order to test the effects of this complete strategy, control animals only received injections with culture medium rostral and caudal to the lesion gap. Anatomically, our multifactorial intervention resulted in an enhanced presence of injured CS axons directly rostral to the lesion gap (65.0 +/- 12.8% in transplanted animals versus 13.1 +/- 3.9% in control animals). No regrowth of these axons was observed through the lesion site, which may be related to a lack of OEC/ONF survival on the biomatrices. Furthermore, a 10-fold increase of neurofilament-positive axon ingrowth into the lesion site as compared to untreated control animals was observed. With the use of quantitative gait analysis, a modest recovery in stride length and swing speed of the hind limbs was observed. Although multifactorial strategies may be needed to stimulate repair of large spinal lesion gaps, we conclude that the combined use of OEC/ONF and poly(D,L)-lactide biomatrices is rather limited.
机译：在大鼠实验性脊髓损伤后，包括嗅鞘细胞（OEC）和嗅神经成纤维细胞（ONF）在内的细胞移植先前已导致切断的皮质脊髓（CS）轴突再生长，穿过小病变间隙并部分恢复功能。为了刺激CS轴突再生跨越较大的病变间隙，我们使用了多因素移植策略在脊髓背侧半切变病变间隙为2毫米长的脊髓中创建了OEC / ONF连续体。该策略涉及在病灶间隙内使用对齐的OEC / ONF-聚（D，L）-丙交酯生物基质桥，以及在病灶间隙的头端和尾端1 mm处注射OEC / ONF。为了测试此完整策略的效果，对照动物仅注射了有病灶间隙和尾状的尾状培养基。从解剖学上讲，我们的多因素干预导致直接位于病变间隙正前方的受损CS轴突的存在（移植动物为65.0 +/- 12.8％，而对照动物为13.1 +/- 3.9％）。通过病变部位未观察到这些轴突的再生，这可能与生物基质上缺乏OEC / ONF存活有关。此外，观察到与未处理的对照动物相比，神经丝阳性轴突向病变部位的向内生长增加了10倍。使用定量步态分析，观察到步幅和后肢摆动速度的适度恢复。尽管可能需要采取多因素策略来刺激大的脊髓病变间隙的修复，但我们得出的结论是，OEC / ONF和聚（D，L）-丙交酯生物基质的联合使用相当有限。

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