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首页> 外文期刊>Experimental dermatology >Development of an in vitro coculture of primary sensitive pig neurons and keratinocytes for the study of cutaneous neurogenic inflammation.
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Development of an in vitro coculture of primary sensitive pig neurons and keratinocytes for the study of cutaneous neurogenic inflammation.

机译：初级敏感猪神经元和角质形成细胞体外共培养的研究，用于研究皮肤神经源性炎症。

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Cutaneous neurogenic inflammation (CNI) is often associated with skin disorders. Activated sensory neurons secrete neuropeptides, such as substance P (SP), which initiate or aggravate inflammation in the skin. The discovery of new molecules acting on these neurons is hampered by the difficulty of reproducing the interactions between nerve endings and skin in vitro. We developed an in vitro model based on the coculture of porcine primary keratinocytes and sensory neurons, which mimics skin innervation. To test the relevance of this model, we compared the effects of different substances on CNI by measuring SP secretion in vitro using a sensitive enzyme immunoassay. Collectively, our results indicate that the use of porcine cells could be very useful to perform an in vitro model of CNI. By adding capsaicin, which induces the secretion of SP by neurons, to the culture, we show that our model mimics CNI in vitro, allowing us to screen for molecules that inhibit this inflammatory response. Such a model can be used to test the effects of different substances on CNI and may be useful for dermatological or cosmetic applications. Based on our screen, we found that extracts of Laminaria digitata and Vernonia sublutea inhibit CNI.
机译：皮肤神经源性炎症（CNI）通常与皮肤疾病有关。激活的感觉神经元分泌神经肽，例如P物质（SP），会引发或加剧皮肤炎症。作用于这些神经元的新分子的发现因难以在体外再现神经末梢与皮肤之间的相互作用而受到阻碍。我们开发了基于猪原代角质形成细胞和感觉神经元的共培养的体外模型，该模型可模拟皮肤神经支配。为了测试该模型的相关性，我们通过使用敏感的酶免疫法体外测量SP分泌，比较了不同物质对CNI的影响。总体而言，我们的结果表明，使用猪细胞对执行CNI体外模型可能非常有用。通过向培养物中添加辣椒素（诱导神经元分泌SP的辣椒素），我们发现我们的模型在体外模拟了CNI，从而使我们能够筛选出抑制这种炎症反应的分子。这样的模型可用于测试不同物质对CNI的影响，并可用于皮肤病学或美容学应用。根据我们的筛选，我们发现海带和紫草提取物可抑制CNI。

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