...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Experimental dermatology >Proteomic profiling reveals a catalogue of new candidate proteins for human skin aging.
【24h】

Proteomic profiling reveals a catalogue of new candidate proteins for human skin aging.

机译：蛋白质组学分析揭示了人类皮肤衰老的新候选蛋白的目录。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Studies of skin aging are usually performed at the genomic level by investigating differentially regulated genes identified through subtractive hybridization or microarray analyses. In contrast, relatively few studies have investigated changes in protein expression of aged skin using proteomic profiling by two-dimensional (2-D) gel electrophoresis and mass spectrometry, although this approach at the protein level is suggested to reflect more accurately the aging phenotype. We undertook such a proteomic analysis of intrinsic human skin aging by quantifying proteins extracted and fluorescently labeled from sun-protected human foreskin samples pooled from 'young' and 'old' men. In addition, we analyzed these candidate gene products by 1-D and 2-D western blotting to obtain corroborative protein expression data, and by both real-time PCR (RT-PCR) and microarray analyses to confirm expression at the mRNA level. We discovered 30 putative proteins for skin aging, including previously unrecognized, post-translationally regulated candidates such as phosphatidyl-ethanolamine binding protein (PEBP) and carbonic anhydrase 1 (CA1).
机译：皮肤衰老的研究通常是在基因组水平上，通过研究通过消减杂交或微阵列分析鉴定的差异调控基因来进行的。相比之下，尽管蛋白质水平的这种方法建议更准确地反映衰老表型，但相对较少的研究已经通过蛋白质组学分析（二维（2-D）凝胶电泳和质谱）研究了老年皮肤蛋白质表达的变化。我们通过量化从“年轻”和“老”男人收集的受防晒保护的人类包皮样品中提取和荧光标记的蛋白质，对人体固有的皮肤老化进行了蛋白质组学分析。此外，我们通过1-D和2-D Western印迹分析了这些候选基因产物，以获得了确证的蛋白表达数据，并通过实时PCR（RT-PCR）和微阵列分析来确认在mRNA水平的表达。我们发现了30种假定的蛋白质可用于皮肤衰老，其中包括以前无法识别的，翻译后调节的候选蛋白，例如磷脂酰乙醇胺结合蛋白（PEBP）和碳酸酐酶1（CA1）。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号