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首页> 外文期刊>Experimental dermatology >Expression of 67-kDa elastin receptor in annular elastolytic giant cell granuloma: elastin peptides induce monocyte-derived dendritic cells or macrophages to form granuloma in vitro.
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Expression of 67-kDa elastin receptor in annular elastolytic giant cell granuloma: elastin peptides induce monocyte-derived dendritic cells or macrophages to form granuloma in vitro.

机译:67 kDa弹性蛋白受体在环状弹性巨细胞肉芽肿中的表达:弹性蛋白肽诱导单核细胞衍生的树突状细胞或巨噬细胞在体外形成肉芽肿。

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Annular elastolytic giant cell granuloma (AEGCG) is characterized by non-palisading granuloma and elastophagocytic giant cells. Granulomas consist of structured masses of macrophages, dendritic cells, and T lymphocytes which play an essential role in granuloma formation. Two lineage systems of dendritic cells and macrophages originated from peripheral blood monocytes have been established in vitro. To know how elastin fragments are involved in the granuloma formation in AEGCG, we tested in vitro whether elastin fragments potentially induce monocyte-derived macrophages or dendritic cells to form granuloma and multinucleated giant cells. Immunohistochemical studies of the lesional skins of AEGCG (n = 5) revealed that the 67-kDa elastin receptor was specifically expressed in the epithelioid or multinucleated giant cells. Proliferation of factor XIIIa(+) cells and CD68(+) cells was also seen in the lesional skins of AEGCG. Factor XIIIa(+) dendritic cells or CD68(+) macrophages were established by the treatment of granulocyte/macrophage-colony stimulating factor (GM-CSF)/interleukin-4 or M-CSF, respectively. Further treatments of these dendritic cells or macrophages with elastin peptide resulted in the formation of granuloma or multinucleated giant cells which were immunoreactive with anti-67-kDa elastin receptor antibody. These findings suggest that elastic tissue induces factor XIIIa(+) cells and CD68(+) macrophages to form granuloma or multinucleated giant cells and plays an essential role in the formation of granuloma in AEGCG.
机译:环形弹性溶解性巨细胞肉芽肿(AEGCG)的特征是非苍白性肉芽肿和弹性吞噬巨细胞。肉芽肿由巨噬细胞,树突状细胞和T淋巴细胞的结构性物质组成,它们在肉芽肿的形成中起重要作用。在体外已经建立了源自外周血单核细胞的树突状细胞和巨噬细胞的两个谱系系统。要了解弹性蛋白片段如何参与AEGCG中肉芽肿的形成,我们在体外测试了弹性蛋白片段是否可能诱导单核细胞衍生的巨噬细胞或树突状细胞形成肉芽肿和多核巨细胞。对AEGCG(n = 5)病变皮肤的免疫组织化学研究表明,67 kDa弹性蛋白受体在上皮样或多核巨细胞中特异性表达。在AEGCG的病变皮肤中还可以看到XIIIa(+)因子细胞和CD68(+)细胞的增殖。通过分别处理粒细胞/巨噬细胞集落刺激因子(GM-CSF)/白细胞介素-4或M-CSF,建立了因子XIIIa(+)树突状细胞或CD68(+)巨噬细胞。用弹性蛋白肽进一步处理这些树突状细胞或巨噬细胞导致形成肉芽肿或多核巨细胞,它们与抗67-kDa弹性蛋白受体抗体发生免疫反应。这些发现表明,弹性组织诱导因子XIIIa(+)细胞和CD68(+)巨噬细胞形成肉芽肿或多核巨细胞,并在AEGCG中肉芽肿的形成中起重要作用。

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