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首页> 外文期刊>Experimental dermatology >Human umbilical cord blood cells form epidermis in the skin equivalent model.
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Human umbilical cord blood cells form epidermis in the skin equivalent model.

机译：人脐带血细胞在皮肤等效模型中形成表皮。

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Recently, human embryonic stem cells have been differentiated in vitro into functional epidermal keratinocytes. Here, we demonstrated that these cells can be generated also from non-embryonic, human umbilical cord blood (hUCB) cells that have the potential to differentiate into cells of non-hematopoietic lineage. Human UCB mono-nucleated cells were cultivated in monolayer and in three-dimensional skin equivalent cultures and assayed for the presence of phenotype-specific markers. Our results determined that after one month of culturing in serum containing medium, the hUCB cells produced morphologically homogeneous colonies of epithelial cells expressing keratinocyte-specific markers. They also formed stratified epidermis in organ cultures that contained sporadic CD1a-positive cells within the accurate strata. We concluded that hUCB cells have the capacity to differentiate into functional epidermal keratinocytes and may serve as a source of high-quality keratinocytes for clinical applications.
机译：最近，人类胚胎干细胞已在体外分化为功能性表皮角质形成细胞。在这里，我们证明了这些细胞也可以由非胚胎人脐带血（hUCB）细胞生成，这些细胞具有分化为非造血细胞谱系的潜能。在单层和三维皮肤等效培养物中培养人UCB单核细胞，并检测表型特异性标记物的存在。我们的结果确定，在含血清的培养基中培养一个月后，hUCB细胞产生表达角质形成细胞特异性标记的上皮细胞形态上均一的菌落。他们还在器官培养物中形成了分层的表皮，在准确的层中包含零星的CD1a阳性细胞。我们得出的结论是，hUCB细胞具有分化为功能性表皮角质形成细胞的能力，并且可以作为临床应用中高质量角质形成细胞的来源。

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