...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Experimental dermatology >Resveratrol regulates p66Shc activation in HaCaT cells.
【24h】

Resveratrol regulates p66Shc activation in HaCaT cells.

机译：白藜芦醇调节HaCaT细胞中p66Shc的活化。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Skin is exposed to both endogenous and environmental oxidant agents, leading to the harmful generation of reactive oxygen species. Particular interest has been pointed on plant antioxidants, such as resveratrol, because of their wide-ranging biological activity and clinical potential. Resveratrol exerts antioxidant, metabolism-regulating and pro-apoptotic/anti-cancer effects on a variety of experimental models and has been suggested to protect skin from ultraviolet-induced photodamaging and photoaging. In parallel, also the biological significance of p66Shc, a member of the Src Homologue and Collagene homologue family with redox activity, is getting further attention. Because of the striking intersection among the activities of resveratrol with those of p66Shc, we investigated whether resveratrol would activate p66Shc in human immortalised keratinocytes (HaCaT cells), a well known and largely used model for skin keratinocytes. HaCaT cells were treated with resveratrol (10-150 mum) for different times. The effect of resveratrol on the proliferation of HaCaT cells and the activation of ERK1/2, AKT, and p66Shc was investigated by cell counting, fluorescence-activated cell sorting, and western blot analysis of total or immunoprecipitated cell extracts. In HaCaT cells, resveratrol induces dose- and time-dependent growth arrest, p66Shc-Ser36 phosphorylation, ERK1/2 phosphorylation and AKT dephosphorylation. Finally, we showed that resveratrol-induced p66Shc-Ser36 phosphorylation is dependent on ERK1/2 activation. Interestingly, these resveratrol-induced molecular effects were associated with reduced adhesion and reversible growth arrest rather than cell death pathways. This is the first evidence linking resveratrol with p66Shc and suggests that p66Shc may contribute to the effect of resveratrol on cell proliferation and function in the outermost layer of the skin.
机译：皮肤接触内源性氧化剂和环境氧化剂，导致有害的活性氧生成。由于其广泛的生物学活性和临床潜力，人们特别关注植物抗氧化剂，例如白藜芦醇。白藜芦醇可在多种实验模型上发挥抗氧化，调节代谢和促凋亡/抗癌作用，并已被建议保护皮肤免受紫外线引起的光损伤和光老化。同时，具有氧化还原活性的Src同源物和胶原蛋白同源物家族成员p66Shc的生物学意义也日益受到关注。由于白藜芦醇的活性与p66Shc的活性之间惊人的交集，我们研究了白藜芦醇是否会激活人类永生化角质形成细胞（HaCaT细胞）中的p66Shc，HaCaT细胞是皮肤角质形成细胞的一种众所周知且被广泛使用的模型。 HaCaT细胞用白藜芦醇（10-150微米）处理不同的时间。通过细胞计数，荧光激活细胞分选以及总或免疫沉淀细胞提取物的蛋白质印迹分析，研究了白藜芦醇对HaCaT细胞增殖以及ERK1 / 2，AKT和p66Shc活化的影响。在HaCaT细胞中，白藜芦醇可诱导剂量和时间依赖性的生长停滞，p66Shc-Ser36磷酸化，ERK1 / 2磷酸化和AKT去磷酸化。最后，我们表明白藜芦醇诱导的p66Shc-Ser36磷酸化依赖于ERK1 / 2激活。有趣的是，这些白藜芦醇诱导的分子效应与粘附减少和可逆的生长停滞有关，而不是与细胞死亡途径有关。这是将白藜芦醇与p66Shc联系起来的第一个证据，表明p66Shc可能有助于白藜芦醇对皮肤最外层细胞增殖和功能的影响。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号