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首页> 外文期刊>Experimental Gerontology >In contrast to lung fibroblasts--no signs of senescence in skin fibroblasts of patients with emphysema.
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In contrast to lung fibroblasts--no signs of senescence in skin fibroblasts of patients with emphysema.

机译：与肺成纤维细胞相反-肺气肿患者的皮肤成纤维细胞没有衰老迹象。

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Smoking is known to be linked to skin ageing and there is evidence for premature senescence of parenchymal lung fibroblasts in emphysema. To reveal whether the emphysema-related changes in cellular phenotype extend beyond the lung, we compared the proliferation characteristics of lung and skin fibroblasts between patients with and without emphysema. Parenchymal lung fibroblasts and skin fibroblasts from the upper torso (thus limiting sun exposure bias) were obtained from patients without, or with mild, or with moderate to severe emphysema undergoing lung surgery. We analysed proliferation rate, population doublings (PD), staining for senescence-associated beta-galactosidase (beta-gal) and gene expression of IGFBP-3 and IGFBP-rP1. Population doubling time of lung fibroblasts differed between control, mild, and moderate to severe emphysema (median (IQR) 29.7(10.0), 33.4(6.1), 44.4(21.2) h; p=0.012) and staining for beta-gal was elevated in moderate to severe emphysema. Compared to control subjects, skin fibroblasts from patients with emphysema did not differ with respect to proliferation rate, PD and beta-gal staining, and showed a lower abundance of mRNA for IGFBP-3 and -rP1 (p<0.05, each). These results suggest that the induction of a senescent fibroblast phenotype by cigarette smoke, as observed in emphysema, primarily occurs in the lung.
机译：吸烟与皮肤老化有关，有证据表明肺气肿中实质肺成纤维细胞过早衰老。为了揭示与肺气肿相关的细胞表型变化是否超出了肺，我们比较了有和没有肺气肿的患者肺和皮肤成纤维细胞的增殖特征。来自上躯干的实质性肺成纤维细胞和皮肤成纤维细胞（因此限制了阳光照射偏向）是从没有或有轻度，中度或重度肺气肿接受肺外科手术的患者中获得的。我们分析了增殖率，群体倍增（PD），衰老相关的β-半乳糖苷酶（β-gal）染色以及IGFBP-3和IGFBP-rP1的基因表达。肺成纤维细胞的群体倍增时间在对照，轻度和中度至重度肺气肿之间有所不同（中位（IQR）29.7（10.0），33.4（6.1），44.4（21.2）h; p = 0.012），β-gal的染色升高中度至重度肺气肿。与对照组相比，肺气肿患者的皮肤成纤维细胞在增殖率，PD和β-gal染色方面无差异，并且IGFBP-3和-rP1的mRNA丰度较低（p均<0.05）。这些结果表明，如在肺气肿中所观察到的，香烟烟雾诱导衰老的成纤维细胞表型主要发生在肺中。

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