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首页> 外文期刊>Gene: An International Journal Focusing on Gene Cloning and Gene Structure and Function >Molecular cloning and sequence analysis of heat shock proteins 70 (HSP70) and 90 (HSP90) and their expression analysis when exposed to benzo(a)pyrene in the clam Ruditapes philippinarum
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Molecular cloning and sequence analysis of heat shock proteins 70 (HSP70) and 90 (HSP90) and their expression analysis when exposed to benzo(a)pyrene in the clam Ruditapes philippinarum

机译：菲律宾蛤仔中热休克蛋白70（HSP70）和90（HSP90）的分子克隆，序列分析及其在苯并（a）py中的表达分析

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摘要

HSP70 and HSP90 are the most important heat shock proteins (HSPs), which play the key roles in the cell as molecular chaperones and may involve in metabolic detoxification. The present research has obtained full-length cDNAs of genes HSP70 and HSP90 from the clam Ruditapes philippinarum and studied the transcriptional responses of the two genes when exposed to benzo(a)pyrene (BaP). The full-length RpHSP70 cDNA was 2336 bp containing a 5' untranslated region (UTR) of 51 bp, a 3' UTR of 335 bp and an open reading frame (ORF) of 1950 bp encoding 650 amino acid residues. The full-length RpHSP90 cDNA was 2839 bp containing a 107-bp 5' UTR, a 554-bp 3' UTR and a 2178-bp ORF encoding 726 amino acid residues. The deduced amino acid sequences of RpHSP70 and RpHSP90 shared the highest identity with the sequences of Paphia undulata, and the phylogenetic trees showed that the evolutions of RpHSP70 and RpHSP90 were almost in accord with the evolution of species. The RpHSP70 and RpHSP90 mRNA expressions were detected in all tested tissues in the adult clams (digestive gland, gill, adductor muscle and mantle) and the highest mRNA expression level was observed in the digestive gland compared to other tissues. Quantitative real-time RT-PCR analysis revealed that mRNA expression levels of the clam RpHSP70,RpHSP90 and other xenobiotic metabolizing enzymes (XMEs) (AhR, DD, GST, GPx) in the digestive gland of R. philippinarum were induced by benzo(a)pyrene (BaP) and the absolute expression levels of these genes showed a temporal and dose-dependent response. The results suggested that RpHSP70 and RpHSP90 were involved in the metabolic detoxification of BaP in the clam R. philippinarum. (C) 2014 Elsevier B.V. All rights reserved.
机译：HSP70和HSP90是最重要的热激蛋白（HSP），它们在细胞中起分子伴侣的作用，并可能参与代谢排毒。本研究已从蛤仔菲律宾蛤中获得了基因HSP70和HSP90的全长cDNA，并研究了当暴露于苯并（a）re（BaP）时这两个基因的转录反应。全长RpHSP70 cDNA为2336 bp，包含51 bp的5'非翻译区（UTR），335 bp的3'UTR和1950 bp的开放阅读框（ORF），编码650个氨基酸残基。全长RpHSP90 cDNA为2839 bp，包含107 bp的5'UTR，554 bp的3'UTR和2178 bp的ORF，编码726个氨基酸残基。推导的RpHSP70和RpHSP90的氨基酸序列与斑叶兜兰（Paphia undulata）的序列具有最高的同一性，系统进化树表明，RpHSP70和RpHSP90的进化与物种的进化基本一致。在成年蛤的所有测试组织（消化腺，腮，内收肌和地幔）中检测到RpHSP70和RpHSP90 mRNA表达，与其他组织相比，在消化腺中观察到最高的mRNA表达水平。实时定量RT-PCR分析显示，蛤phil RpHSP70，RpHSP90和其他异生代谢酶（XME）（AhR，DD，GST，GPx）的mRNA表达水平是由苯并（a）诱导的。 py（BaP）和这些基因的绝对表达水平显示出时间和剂量依赖性反应。结果表明，RpHSP70和RpHSP90参与了菲律宾蛤仔BaP的代谢解毒。 （C）2014 Elsevier B.V.保留所有权利。

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